Biomaterial Database

Determination of the complete amino acid sequence of protein S21 from Escherichia coli ribosomes. 1975

J Vandekerckhove, and W Rombauts, and B Peeters, and B Wittmann-Liebold

Protein S21 was digested with trypsin before and after maleylation, with chymotrypsin, thermolysin and a glutamyl-specific protease. The resulting peptides were isolated and their amino acid composition determined. The amino acid sequences of selected peptides were determined either by the manual subtractive Edman method or by the dansyl-Edman procedure. Additional information was obtained from the automatic Edman degradation of the whole protein in a modified Sequenator. All these results combined yielded the sequence shown in Fig. 1. Protein S21 consists of 70 amino acids (Asp1,Asn2,Thr3,Ser2,Glu8,Pro3,Gly1,Ala9,Val6,Cys1,Ile1,Leu4,Tyr2,Phe3,His1,Lys9 and Arg14). It contains neither Met nor Trp. The molecular weight amounts to 8359. Clustering of basic amino acids is observed in five regions. We also include a prediction for regions with alpha-helices and with beta-sheets.

UI	MeSH Term	Description	Entries
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino
D001426	Bacterial Proteins	Proteins found in any species of bacterium.	Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D012269	Ribosomal Proteins	Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits.	Proteins, Ribosomal,Ribosomal Protein,Protein, Ribosomal