Biomaterial Database

Evaluation of an agar gel immunodiffusion test to detect infection of cattle with bluetongue viruses in Queensland, Australia. 1995

M P Ward, and I A Gardner, and M Flanagan

Queensland Department of Primary Industries, Toowoomba, Australia.

An agar gel immunodiffusion (AGID) test to detect group-specific antibodies to infection of cattle by bluetongue viruses was evaluated using field collected sera in Queensland, Australia. The AGID test was compared to the serum neutralisation (SN) test used to detect serotype-specific bluetongue virus antibodies. The AGID test was found to be highly sensitive (95% confidence interval, 80.7-100%) but to have moderate specificity (95% confidence interval, 59.3-79.6%), relative to the SN test. The correlation between AGID and SN test results was 0.41 (P < 0.001). Using likelihood ratio estimates the chance (posttest probability) of an AGID test positive serum sample being positive (titre > or = 1:20) in the SN test was estimated to be 87% and 90% for 2+ and 3+ AGID test reactions, respectively. A serum sample was estimated to have a probability of 40% of being positive in the SN test if it gave a 1+ reaction in the AGID test. Results suggest that the AGID test should ideally be used as a screening test in serological surveys and surveillance programs. If the test is to be used for identification of individually infected cattle, a cutoff point of > or = 2+ should be employed.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010865	Pilot Projects	Small-scale tests of methods and procedures to be used on a larger scale if the pilot study demonstrates that these methods and procedures can work.	Pilot Studies,Pilot Study,Pilot Project,Project, Pilot,Projects, Pilot,Studies, Pilot,Study, Pilot
D011793	Queensland	A state in northeastern Australia. Its capital is Brisbane. Its coast was first visited by Captain Cook in 1770 and its first settlement (penal) was located on Moreton Bay in 1824. The name Cooksland was first proposed but honor to Queen Victoria prevailed. (From Webster's New Geographical Dictionary, 1988, p996 & Room, Brewer's Dictionary of Names, 1992, p441)
D001819	Bluetongue	A reovirus infection, chiefly of sheep, characterized by a swollen blue tongue, catarrhal inflammation of upper respiratory and gastrointestinal tracts, and often by inflammation of sensitive laminae of the feet and coronet.	Blue Tongue,Tongue, Blue
D001820	Bluetongue virus	The type species of ORBIVIRUS causing a serious disease in sheep, especially lambs. It may also infect wild ruminants and other domestic animals.	Ovine Catarrhal Fever Virus,Blue Tongue Virus,Blue Tongue Viruses,Bluetongue Viruses
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002418	Cattle Diseases	Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.	Bovine Diseases,Bovine Disease,Cattle Disease,Disease, Bovine,Disease, Cattle,Diseases, Bovine,Diseases, Cattle
D005260	Female		Females
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia