-transparent.png){kind=logo}
The gene encoding the complete glycoprotein gII (homologue of gB of herpes simplex virus) of pseudorabies virus (PrV) was inserted into a baculovirus transfer vector, and a recombinant virus expressing gII was isolated. Three gII-related recombinant baculovirus-expressed peptides of 100, 60, and 45 to 50 kDa were detected with a polyclonal antibody against gII; these correspond to the authentic subunits gIIa and its cleavage products gIIb and gIIc, respectively. These proteins were subjected to N-terminal sequencing, and the results showed that the protease cleavage sites were identical to those of authentic gII. The expressed gII was shown to be transported to the surface of infected cells as judged by an indirect immunofluorescence test. Antibodies raised in mice immunized with the recombinant gII neutralized the infection of PrV in vitro. Mice inoculated with the recombinant gII were completely protected from lethal challenge with PrV.
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
September 1992,
Virus research,
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
June 1990,
Journal of virology,
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
June 1991,
Virus research,
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
October 1999,
The Journal of veterinary medical science,
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
July 1994,
Virology,
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
January 2006,
Acta virologica,
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
December 1996,
Virus research,
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
August 1999,
Veterinary microbiology,
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
July 1986,
Virology,
X Xuan, and
T Nakamura, and
T Ihara, and
I Sato, and
K Tuchiya, and
E Nosetto, and
A Ishihama, and
S Ueda
April 1994,
Journal of virology,
