-transparent.png){kind=logo}
A stable Chinese hamster ovary fibroblast line expressing the rat vascular type 1a angiotensin II (ANG II) receptor was used to study the lipid-derived signal transduction pathways elicited by type 1a ANG II receptor activation. ANG II caused a biphasic and dose-dependent increase in diacylglycerol (DAG) accumulation with an initial peak at 15 s (181 +/- 11% of control, P < 0.02) and a second sustained peak at 5-10 min (214 +/- 10% of control, P < 0.02). The late DAG peak was derived from phosphatidylcholine (PC), and the formation was blocked by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid. ANG II also increased phosphatidic acid (PA) production nearly fourfold by 7.5 min. In the presence of ethanol, ANG II markedly increased phosphatidylethanol (PEt) formation, indicating activation of phospholipase D (PLD). ANG II was shown to increase the mass of three separate PA species, one of which apparently originated from DAG kinase action on PC-phospholipase C (PLC)-produced DAG, providing evidence for PC-PLC activity. ANG II also formed a third PA species, which originated neither from PLD nor from DAG kinase. These results demonstrate that multiple lipid signals propagated via collateral stimulation of PLC and PLD are generated by specific activation of the vascular type 1a ANG II receptor.
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
November 1998,
Regulatory peptides,
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
July 2000,
Regulatory peptides,
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
April 2002,
Current hypertension reports,
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
March 1994,
Biochemical and biophysical research communications,
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
January 1996,
Basic research in cardiology,
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
May 2014,
Experimental cell research,
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
January 2001,
Contributions to nephrology,
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
January 1994,
Trends in cardiovascular medicine,
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
October 1990,
The Journal of biological chemistry,
Y Wen, and
M C Cabot, and
E Clauser, and
S L Bursten, and
J L Nadler
November 2002,
Journal of cardiovascular pharmacology,
