Biomaterial Database

Matrix-assisted laser desorption/ionization mass spectrometry: improved matrix for oligosaccharides. 1995

M D Mohr, and K O Börnsen, and H M Widmer

Corporate Analytical Research, Ciba Geigy Ltd., Basel, Switzerland.

The aim of this article was to study the influence of different matrix molecules on the quality of matrix-assisted laser desorption/ionization mass spectra of oligosaccharides. An important criterion was the sample preparation, i.e. the crystallization process leading to the matrix from which the analytes were desorbed and investigated. Quality criteria were, among others, the resulting molecular peak intensity, the mass resolution, and the suppression of unwanted matrix peak. It was found that a mixture of 2,5-dihydroxy benzoic acid (DHB) and 1-hydroxy isoquinoline (HIC) in a weight ratio of 3:1 was best suited for the analytical investigation of oligosaccharides. In addition, this matrix mixture was found to be quite tolerant against all kinds of buffers, salts, and even additives such as sodium dodecyl sulfate (SDS). Furthermore, we determined the different affinities of the alkaline metals to the carbohydrates and found that cesium and potassium ions ionize oligosaccharides about three times better than sodium ions and therefore have an important influence on the quantum yield.

UI	MeSH Term	Description	Entries
D007834	Lasers	An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.	Masers,Continuous Wave Lasers,Pulsed Lasers,Q-Switched Lasers,Continuous Wave Laser,Laser,Laser, Continuous Wave,Laser, Pulsed,Laser, Q-Switched,Lasers, Continuous Wave,Lasers, Pulsed,Lasers, Q-Switched,Maser,Pulsed Laser,Q Switched Lasers,Q-Switched Laser
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009844	Oligosaccharides	Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.	Oligosaccharide
D002240	Carbohydrate Sequence	The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.	Carbohydrate Sequences,Sequence, Carbohydrate,Sequences, Carbohydrate
D005504	Food Analysis	Measurement and evaluation of the components of substances to be taken as FOOD.	Analysis, Food,Analyses, Food,Food Analyses
D013058	Mass Spectrometry	An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.	Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass