Biomaterial Database

Localization of the gene for human 11 beta-hydroxysteroid dehydrogenase type 2 (HSD11B2) to chromosome band 16q22. 1995

Z Krozowski, and E Baker, and V Obeyesekere, and D F Callen

Baker Institute for Medical Research, Prahran, Victoria, Australia.

The enzyme 11 beta-hydroxysteroid dehydrogenase type 2 (11 beta HSD2) is an NAD-dependent, high-affinity isoform that potently inactivates glucocorticoids. In the present study we have used fluorescence in situ hybridization and an 11 beta HSD2 cDNA isolated from human kidney as probe to localize the gene encoding 11 beta HSD2. The gene, which has been given the symbol HSD11B2, maps to human chromosome band 16q22.

UI	MeSH Term	Description	Entries
D008677	Metaphase	The phase of cell nucleus division following PROMETAPHASE, in which the CHROMOSOMES line up across the equatorial plane of the SPINDLE APPARATUS prior to separation.
D002885	Chromosomes, Human, Pair 16	A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.	Chromosome 16
D002999	Clone Cells	A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)	Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006913	Hydroxysteroid Dehydrogenases	Enzymes of the oxidoreductase class that catalyze the dehydrogenation of hydroxysteroids. (From Enzyme Nomenclature, 1992) EC 1.1.-.	Hydroxysteroid Dehydrogenase,Dehydrogenase, Hydroxysteroid,Dehydrogenases, Hydroxysteroid
D015342	DNA Probes	Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.	Chromosomal Probes,DNA Hybridization Probe,DNA Probe,Gene Probes, DNA,Conserved Gene Probes,DNA Hybridization Probes,Whole Chromosomal Probes,Whole Genomic DNA Probes,Chromosomal Probes, Whole,DNA Gene Probes,Gene Probes, Conserved,Hybridization Probe, DNA,Hybridization Probes, DNA,Probe, DNA,Probe, DNA Hybridization,Probes, Chromosomal,Probes, Conserved Gene,Probes, DNA,Probes, DNA Gene,Probes, DNA Hybridization,Probes, Whole Chromosomal
D017404	In Situ Hybridization, Fluorescence	A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.	FISH Technique,Fluorescent in Situ Hybridization,Hybridization in Situ, Fluorescence,FISH Technic,Hybridization in Situ, Fluorescent,In Situ Hybridization, Fluorescent,FISH Technics,FISH Techniques,Technic, FISH,Technics, FISH,Technique, FISH,Techniques, FISH
D042842	11-beta-Hydroxysteroid Dehydrogenases	Hydroxysteroid dehydrogenases that catalyzes the reversible conversion of CORTISOL to the inactive metabolite CORTISONE. Enzymes in this class can utilize either NAD or NADP as cofactors.	11 beta-Hydroxysteroid Dehydrogenase,11-Hydroxysteroid Dehydrogenase,11B-Hydroxysteroid Dehydrogenase,11beta-Hydroxysteroid Dehydrogenase,Corticosteroid 11-Oxidoreductase,Corticosteroid 11-Reductase,Cortisone 11-Oxoreductase,11 Hydroxysteroid Dehydrogenase,11 beta Hydroxysteroid Dehydrogenase,11 beta Hydroxysteroid Dehydrogenases,11-Oxidoreductase, Corticosteroid,11-Oxoreductase, Cortisone,11-Reductase, Corticosteroid,11B Hydroxysteroid Dehydrogenase,11beta Hydroxysteroid Dehydrogenase,Corticosteroid 11 Oxidoreductase,Corticosteroid 11 Reductase,Cortisone 11 Oxoreductase,Dehydrogenase, 11 beta-Hydroxysteroid,Dehydrogenase, 11-Hydroxysteroid,Dehydrogenase, 11B-Hydroxysteroid,Dehydrogenase, 11beta-Hydroxysteroid,Dehydrogenases, 11-beta-Hydroxysteroid,beta-Hydroxysteroid Dehydrogenase, 11