BIOMAT Database Logo BIOMAT Database Logo

Isolation and characterization of metastatic variants from human transitional cell carcinoma passaged by orthotopic implantation in athymic nude mice. 1995

C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.

OBJECTIVE These studies were designed to develop an orthotopic model for human bladder cancer and to isolate variant metastatic cell lines. METHODS The human bladder cancer cell line 253J was implanted into the muscular wall of the bladder of athymic nude mice. By in vivo recycling, we selected for 2 variant cell lines: 253J B-V, a bladder line isolated after 5 serial passages in the bladder, and 253J lung-IV, established from a lung tumor nodule that was recycled through the bladder. RESULTS These 2 cell lines showed enhanced tumorigenicity, as measured by a decreased latent period, and rapid growth as compared with the parental cell line. Moreover, orthotopic implantation of these cell lines resulted in metastasis to the lungs. These in vivo-selected, metastatic cell lines exhibited unique karyotypic alterations, increased anchorage-independent growth, overexpression of basic fibroblast growth factor, altered expression of adhesion molecules and the ability to migrate through Matrigel. CONCLUSIONS This reproducible model of human bladder cancer offers the opportunity to study cellular properties associated with tumor progression and metastasis and is suitable for the evaluation of new therapeutic strategies for invasive bladder cancer.

UI MeSH Term Description Entries
D007621 Karyotyping Mapping of the KARYOTYPE of a cell. Karyotype Analysis Methods,Analysis Method, Karyotype,Analysis Methods, Karyotype,Karyotype Analysis Method,Karyotypings,Method, Karyotype Analysis,Methods, Karyotype Analysis
D008819 Mice, Nude Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses. Athymic Mice,Mice, Athymic,Nude Mice,Mouse, Athymic,Mouse, Nude,Athymic Mouse,Nude Mouse
D009368 Neoplasm Transplantation Experimental transplantation of neoplasms in laboratory animals for research purposes. Transplantation, Neoplasm,Neoplasm Transplantations,Transplantations, Neoplasm
D001749 Urinary Bladder Neoplasms Tumors or cancer of the URINARY BLADDER. Bladder Cancer,Bladder Neoplasms,Cancer of Bladder,Bladder Tumors,Cancer of the Bladder,Malignant Tumor of Urinary Bladder,Neoplasms, Bladder,Urinary Bladder Cancer,Bladder Cancers,Bladder Neoplasm,Bladder Tumor,Cancer, Bladder,Cancer, Urinary Bladder,Neoplasm, Bladder,Neoplasm, Urinary Bladder,Tumor, Bladder,Tumors, Bladder,Urinary Bladder Neoplasm
D002295 Carcinoma, Transitional Cell A malignant neoplasm derived from TRANSITIONAL EPITHELIAL CELLS, occurring chiefly in the URINARY BLADDER; URETERS; or RENAL PELVIS. Carcinomas, Transitional Cell,Cell Carcinoma, Transitional,Cell Carcinomas, Transitional,Transitional Cell Carcinoma,Transitional Cell Carcinomas
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D015972 Gene Expression Regulation, Neoplastic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue. Neoplastic Gene Expression Regulation,Regulation of Gene Expression, Neoplastic,Regulation, Gene Expression, Neoplastic

Related Publications

C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
New orthotopic implantation model of human esophageal squamous cell carcinoma in athymic nude mice.
September 2014, Thoracic cancer,
C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
In vivo selection and characterization of metastatic variants from human pancreatic adenocarcinoma by using orthotopic implantation in nude mice.
April 1999, Neoplasia (New York, N.Y.),
C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
The importance of orthotopic implantation to the isolation and biological characterization of a metastatic human clear cell renal-carcinoma in nude-mice.
January 1993, International journal of oncology,
C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
Isolation of a highly metastatic cell line to lymph node in human oral squamous cell carcinoma by orthotopic implantation in nude mice.
July 1998, Oral oncology,
C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
Metastatic potential of human renal cell carcinoma: experimental model using subrenal capsule implantation in athymic nude mice.
January 1992, Urological research,
C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
Selection of highly metastatic variants of different human prostatic carcinomas using orthotopic implantation in nude mice.
September 1996, Clinical cancer research : an official journal of the American Association for Cancer Research,
C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
Microscopic metastasis of a human lung carcinoma cell line in athymic nude mice: isolation of a metastatic variant.
July 1987, International journal of cancer,
C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
[Metastatic characteristics of SY86B human gastric carcinoma in athymic nude mice].
November 1988, Zhonghua zhong liu za zhi [Chinese journal of oncology],
C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
Heterotransplantation of human transitional cell carcinoma in athymic mice.
February 1979, The Journal of urology,
C P Dinney, and R Fishbeck, and R K Singh, and B Eve, and S Pathak, and N Brown, and B Xie, and D Fan, and C D Bucana, and I J Fidler
Development of a metastatic model for human endometrial carcinoma using orthotopic implantation in nude-mice.
June 1994, International journal of oncology,
Copied contents to your clipboard!