Biomaterial Database

Immunoblot analysis using recombinant protein of the 180 kD bullous pemphigoid antigen NC16a domain as an aid to the diagnosis of atypical subepidermal autoimmune bullous skin diseases. 1995

T Hashimoto, and K Matsumura, and Y Kawahara, and Y Ohata, and T Nishikawa

Department of Dermatology, School of Medicine, Keio University, Tokyo, Japan.

We have recently developed a novel immunoblotting system using bacterial fusion protein containing the NC16a domain, the most immunogenic region, of the 180 kD bullous pemphigoid (BP) antigen, which was shown to be highly sensitive and specific method to detect the antigen and to be very useful for the diagnosis of BP. In this study, using this immunoblot assay, we investigated sera obtained from 26 patients who showed atypical clinical features. The sera of these cases showed in general very weak reactivity with the basement membrane zone on immunofluorescence, and very weak and variable results in detection of either the 230 kD or 180 kD BP antigen with immunoblotting of human epidermal extracts. However, with immunoblotting of the BP180 NC16a domain fusion protein, this protein was detected by 21 out of the 26 cases. These results indicated that this new assay system is the most sensitive method to detect BP antigen, and should be very useful for the diagnosis for these atypical cases.

UI	MeSH Term	Description	Entries
D009419	Nerve Tissue Proteins		Proteins, Nerve Tissue,Tissue Proteins, Nerve
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011251	Pregnancy Complications, Infectious	The co-occurrence of pregnancy and an INFECTION. The infection may precede or follow FERTILIZATION.	Complications, Infectious Pregnancy,Infectious Pregnancy Complications,Maternal Sepsis,Pregnancy, Infectious Complications,Sepsis during Pregnancy,Sepsis in Pregnancy,Infectious Pregnancy Complication,Pregnancy Complication, Infectious,Sepsis in Pregnancies,Sepsis, Maternal
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D003598	Cytoskeletal Proteins	Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.	Proteins, Cytoskeletal
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000071821	Dystonin	A plakin characterized by repeat sequences homologous to SPECTRIN and PLECTIN and C-terminal EF HAND MOTIFS. It functions as an integrator of INTERMEDIATE FILAMENTS, ACTIN and MICROTUBULES in cytoskeleton networks. It is required for anchoring intermediate filaments to the actin cytoskeleton in neural and muscle cells as well as anchoring KERATIN-containing intermediate filaments to HEMIDESMOSOMES in EPITHELIAL CELLS.	230 kDa Bullous Pemphigoid Antigen,230-240 kDa Bullous Pemphigoid Antigen,Bullous Pemphigoid Antigen 1,Hemidesmosomal Plaque Protein,230 240 kDa Bullous Pemphigoid Antigen
D000097805	Collagen Type XVII	A 220 kDa non-fibrillar collagen and basement membrane component. It is involved in healing wounds of the EPITHELIUM, CORNEAL.	180 kDa Bullous Pemphigoid Autoantigen,BP180 Antigen,BP180 Autoantigen,Bullous Pemphigoid Autoantigen, BP180,Collagen Alpha-1(XVII) Chain,Collagen XVII,Collagen, Type XVII, Alpha 1 Chain,Type XVII Collagen
D001324	Autoantigens	Endogenous tissue constituents with the ability to interact with AUTOANTIBODIES and cause an immune response.	Autoantigen,Autologous Antigen,Autologous Antigens,Self-Antigen,Self-Antigens,Antigen, Autologous,Antigens, Autologous,Self Antigen,Self Antigens