Biomaterial Database

Production of aflatoxins B1, B2, G1, and G2 in pure and mixed cultures of Aspergillus parasiticus and Aspergillus flavus. 1995

D M Wilson, and J K King

Department of Plant Pathology, University of Georgia, Tifton 31793, USA.

Production of aflatoxins B1, B2, G1, and G2 in pure and mixed cultures of Aspergillus parasiticus and aspergillus flavus were compared. Differing percentages of A. parasiticus (NRRL 2999) and A. flavus (NRRL 5520) conidia were used as inoculum and allowed to grow in static liquid culture for 10 days. The pure and mixed cultures of A. parasiticus/A. flavus were extracted and the aflatoxins partially purified. High-performance liquid chromatography was then used to determine aflatoxin concentrations. The maximal decreases of G1 and G2 production occurred when the inocula went from 0 to 12.5% or 25% A. flavus. Aflatoxins G1 and G2 comprised less than 10% of the total aflatoxins when the A. parasiticus/A. flavus inocula contained greater than 25% A. flavus. Production of aflatoxins B1 and B2 increased slightly as the percentage of A. flavus in the A. parasiticus/A. flavus mixed inoculum increased. A. flavus is apparently capable of suppressing accumulation of aflatoxins G1 and G2 by A. parasiticus when these fungi are grown in mixed culture.

UI	MeSH Term	Description	Entries
D009172	Mycology	The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.
D000348	Aflatoxins	Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2.	Aflatoxin
D001230	Aspergillus	A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
D001231	Aspergillus flavus	A species of imperfect fungi which grows on peanuts and other plants and produces the carcinogenic substance aflatoxin. It is also used in the production of the antibiotic flavicin.	Aspergillus parvisclerotigenus,Petromyces flavus
D016604	Aflatoxin B1	A potent hepatotoxic and hepatocarcinogenic mycotoxin produced by the Aspergillus flavus group of fungi. It is also mutagenic, teratogenic, and causes immunosuppression in animals. It is found as a contaminant in peanuts, cottonseed meal, corn, and other grains. The mycotoxin requires epoxidation to aflatoxin B1 2,3-oxide for activation. Microsomal monooxygenases biotransform the toxin to the less toxic metabolites aflatoxin M1 and Q1.	Aflatoxin B(1),Aflatoxin B,Aflatoxin B1 Dihydrochloride, (6aR-cis)-Isomer,Aflatoxin B1, (6aR-cis)-Isomer, 14C-Labeled,Aflatoxin B1, (6aR-cis)-Isomer, 2H-Labeled,Aflatoxin B1, (6aR-cis)-Isomer, 3H-Labeled,Aflatoxin B1, cis(+,-)-Isomer,HSDB-3453,NSC-529592,HSDB 3453,HSDB3453,NSC 529592,NSC529592