-transparent.png){kind=logo}
Chromosomal rearrangements of BCL6 are commonly associated with diffuse large-cell lymphomas. We set out to determine the DNA-binding site of a glutathione-S-transferase fusion protein containing the BCL6 zinc finger region by employing cyclic amplification and selection of targets (CASTing). From oligonucleotides containing 16 central random bases, sequences binding to the protein on glutathione-coated beads were repeatedly selected and amplified by polymerase chain reaction (PCR). The binding sites were cloned and sequenced. A consensus, TTTNNNGNNATNCTTT, was obtained. Protein binding studies of double-stranded oligomers containing point mutations within the 3' CTTT confirmed the binding specificity of this part of the consensus. In addition, evidence indicated that some of the base pairs held constant in the oligonucleotides used for CASTing also contributed to binding.
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
March 1995,
FEBS letters,
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
January 2011,
PloS one,
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
April 1993,
FEBS letters,
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
October 1988,
Nature,
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
May 1991,
Molecular and cellular biology,
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
April 1992,
Oncogene,
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
January 1985,
Nature,
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
June 1995,
Plant molecular biology,
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
October 1992,
The EMBO journal,
B W Baron, and
R R Stanger, and
E Hume, and
A Sadhu, and
R Mick, and
J P Kerckaert, and
C Deweindt, and
C Bastard, and
G Nucifora, and
N Zeleznik-Le
July 1990,
The Journal of biological chemistry,
