BIOMAT Database Logo BIOMAT Database Logo

Purification of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi by affinity chromatography. 1993

C Labriola, and M Sousa, and J J Cazzulo
Instituto de Investigaciones Bioquímicas Luis F. Leloir, Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CONICET, Argentina.

The major cysteine proteinase from Trypanosoma cruzi, cruzipain, can be obtained essentially homogeneous, starting from crude epimastigote extracts, in one step, by affinity chromatography on Cystatin-Sepharose (specific for cysteine proteinases) or ConA-Sepharose (specific for high mannose N-linked glycoproteins). The methods offer considerable potential for enzyme purification from scarce sources, such as other parasite stages or radioactively labelled material with high specific radioactivity.

UI MeSH Term Description Entries
D002846 Chromatography, Affinity A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D003208 Concanavalin A A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
D003546 Cysteine Endopeptidases ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012685 Sepharose Agarose,Sepharose 4B,Sepharose C1 4B,4B, Sepharose C1,C1 4B, Sepharose
D014349 Trypanosoma cruzi The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors. Trypanosoma cruzus,cruzi, Trypanosoma
D015800 Protozoan Proteins Proteins found in any species of protozoan. Proteins, Protozoan
D015891 Cystatins A homologous group of endogenous CYSTEINE PROTEINASE INHIBITORS. The cystatins inhibit most CYSTEINE ENDOPEPTIDASES such as PAPAIN, and other peptidases which have a sulfhydryl group at the active site. Cystatin,Cystatin Superfamily,Stefin,Cystatin-Related Proteins,Stefins,Type 1 Cystatins,Type 2 Cystatins,Type 3 Cystatins,Type I Cystatins,Type II Cystatins,Type III Cystatins,Cystatin Related Proteins,Cystatins, Type 1,Cystatins, Type 2,Cystatins, Type 3,Cystatins, Type I,Cystatins, Type II,Cystatins, Type III

Related Publications

C Labriola, and M Sousa, and J J Cazzulo
Cruzipain, the major cysteine proteinase from the protozoan parasite Trypanosoma cruzi.
January 1997, Biological chemistry,
C Labriola, and M Sousa, and J J Cazzulo
Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi.
August 1990, FEMS microbiology letters,
C Labriola, and M Sousa, and J J Cazzulo
The C-terminal extension of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi.
April 1991, Molecular and biochemical parasitology,
C Labriola, and M Sousa, and J J Cazzulo
Nitric oxide inhibits cruzipain, the major papain-like cysteine proteinase from Trypanosoma cruzi.
April 2000, Biochemical and biophysical research communications,
C Labriola, and M Sousa, and J J Cazzulo
The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is antigenic in human infections.
November 1991, Infection and immunity,
C Labriola, and M Sousa, and J J Cazzulo
Kininogenase activity by the major cysteinyl proteinase (cruzipain) from Trypanosoma cruzi.
October 1997, The Journal of biological chemistry,
C Labriola, and M Sousa, and J J Cazzulo
Specific cleavage sites on human IgG subclasses by cruzipain, the major cysteine proteinase from Trypanosoma cruzi.
August 2003, Molecular and biochemical parasitology,
C Labriola, and M Sousa, and J J Cazzulo
Some kinetic properties of a cysteine proteinase (cruzipain) from Trypanosoma cruzi.
February 1990, Biochimica et biophysica acta,
C Labriola, and M Sousa, and J J Cazzulo
Modulation of the catalytic activity of cruzipain, the major cysteine proteinase from Trypanosoma cruzi, by temperature and pH.
June 2001, European journal of biochemistry,
C Labriola, and M Sousa, and J J Cazzulo
Degradation of oxidised insulin A and B chains by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi epimastigotes.
December 1991, Molecular and biochemical parasitology,
Copied contents to your clipboard!