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We studied sex determination from dental calculus by the PCR method using primers which recognize DYZ3 region of Y-chromosome and DXZ1 of X-chromosome. The amounts of DNA obtained from 1.7-25.0 mg of dental calculus samples ranged 0.6-26.4 micrograms, and the majority of DNA was low molecular weight DNA. After PCR amplification, Y and X products were observed by the method of polyacrylamide gel electrophoresis. Both Y and X amplification products were observed in 26 out of 29 male samples, while, Y products was not detected in two samples and neither Y nor X product was detected in one sample. In 17 out of 20 female samples, only X product was observed, while, Y and X products were observed in three samples. The minimum amount of DNA for sex determination was 3 pg. Sex determination using DNA in dental calculus will be quite useful for forensic application because it can be done without destruction of morphological characteristics of teeth.
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
February 1994,
Nihon hoigaku zasshi = The Japanese journal of legal medicine,
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
January 1996,
Science & justice : journal of the Forensic Science Society,
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
January 2018,
Indian journal of dental research : official publication of Indian Society for Dental Research,
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
February 1993,
Journal of assisted reproduction and genetics,
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
June 1995,
Medizinische Monatsschrift fur Pharmazeuten,
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
January 2018,
Journal of forensic dental sciences,
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
June 2019,
Cold Spring Harbor protocols,
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
February 2003,
Zygote (Cambridge, England),
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
March 2012,
Animal reproduction science,
S Kawano, and
T Tsukamoto, and
H Ohtaguro, and
H Tsutsumi, and
T Takahashi, and
I Miura, and
R Mukoyama, and
H Aboshi, and
T Komuro
February 2019,
Cold Spring Harbor protocols,
