-transparent.png){kind=logo}
We have developed a set of cell lines to help distinguish the sequelae of specific lesions in DNA after UV irradiation. Irradiation results in two primary lesions: cyclobutane dimers and pyrimidine-pyrimidone (6-4) photoproducts. The contributions of each to mutation are considered utilizing a spectrum of cell lines with increasing abilities to repair these lesions. In particular, we focus on a revertant of the XP12Ro(M1) cell line from a patient with Xeroderma pigmentosum, XP129, which is capable of repairing (6-4) photoproducts but not cyclobutane dimers. We have successfully introduced the denV gene into these cells which confers the ability to repair cyclobutane dimers. By comparing the results of a shuttle vector mutation experiment with the vector pZ189, we can correlate specific mutations to specific lesions.
A P Green, and
J K deRiel, and
E Henderson
September 1987,
Molecular and cellular biology,
A P Green, and
J K deRiel, and
E Henderson
November 1987,
Mutation research,
A P Green, and
J K deRiel, and
E Henderson
May 1973,
Harefuah,
A P Green, and
J K deRiel, and
E Henderson
March 2001,
Lancet (London, England),
A P Green, and
J K deRiel, and
E Henderson
February 1993,
Molecular and cellular biology,
A P Green, and
J K deRiel, and
E Henderson
April 1984,
Experimental cell research,
A P Green, and
J K deRiel, and
E Henderson
July 1977,
Proceedings of the National Academy of Sciences of the United States of America,
A P Green, and
J K deRiel, and
E Henderson
June 1984,
Carcinogenesis,
A P Green, and
J K deRiel, and
E Henderson
February 1998,
Mutation research,
A P Green, and
J K deRiel, and
E Henderson
March 1987,
Mutation research,
