-transparent.png){kind=logo}
The effects of acidic and basic fibroblast growth factors (FGFs) on collagen expression by keloid fibroblasts were examined in the absence and presence of heparin. Collagen biosynthesis and gene expression of type I collagen were down-regulated by the FGFs in the presence of heparin. Acidic FGF, in a concentration range of 0.4 to 50 ng/ml, had little or no effect on collagen synthesis after a 4-day incubation. However, in the presence of heparin (100 micrograms/ml) acidic FGF, in concentrations ranging from 2 to 50 ng/ml, decreased [3H]hydroxyproline synthesis by 44 to 68%, compared with untreated control cultures. Total [3H]hydroxyproline synthesis was similar between control and heparin-treated cultures. Basic FGF (2.0 to 50 ng/ml) was effective in suppressing [3H]hydroxyproline synthesis by 50 to 90% after a 4-day incubation without heparin in keloid and normal fibroblast cultures. The steady-state levels of type I collagen messenger RNA were significantly decreased by acidic FGF in the presence of heparin, as well as by basic FGF without heparin. The data suggest that the FGFs are effective in down-regulating excess collagen production by keloid fibroblasts and that this inhibitory effect is apparently associated with pretranslational events. Moreover, acidic FGF is apparently dependent on heparin, whereas basic FGF is not, for potentiation of the down-regulatory effects of the FGFs.
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
September 1991,
The Biochemical journal,
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
January 1994,
The American journal of pathology,
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
April 2017,
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society,
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
January 1994,
European journal of cancer (Oxford, England : 1990),
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
June 2006,
Acta oto-laryngologica,
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
November 1989,
FEBS letters,
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
January 1991,
Science (New York, N.Y.),
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
May 1995,
Proceedings of the National Academy of Sciences of the United States of America,
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
April 1991,
Proceedings of the National Academy of Sciences of the United States of America,
E M Tan, and
S Rouda, and
S S Greenbaum, and
J H Moore, and
J W Fox, and
S Sollberg
October 1991,
Cancer research,
