-transparent.png){kind=logo}
We have shown that a heptapeptide which resides in the middle part of vitronectin (VN) is responsible for binding to plasminogen activator inhibitor-1 (PAI-1). A single PAI-1 binding peptide was isolated from human VN after limited proteolysis with protease V8. The amino acid sequence of the fragment corresponded to residues Gly-115-Glu-121 of VN. A murine monoclonal antibody (JYV-1) raised against human VN bond to the same fragment and inhibited binding of PAI-1 to VN. A synthetic peptide (V-115), comprising residues Gly-115-Glu-121 of human VN, competed with VN for both PAI-1 and JYV-1 in a dose-dependent manner. Synthetic peptide V-111 (Ser-111-Glu-121) had a stronger inhibitory effect than V-115 on binding of PAI-1 or JYV-1 to VN. V-111 also inhibited the binding of human PAI-1 to mouse and rabbit VN. The functional half-life of PAI-1 activity was prolonged approximately 2-fold in the presence of V-111 (1 mM). This stabilizing effect of V-111 was equivalent to intact VN, although a 1000-fold higher molar concentration of V-111 over VN was required. These data indicated that VN residues Gly-115-Glu-121 contain a PAI-1 binding site.
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
May 1994,
The Journal of biological chemistry,
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
May 1995,
Thrombosis and haemostasis,
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
October 1994,
FEBS letters,
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
July 1991,
FEBS letters,
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
December 2004,
American journal of respiratory cell and molecular biology,
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
April 1989,
The Journal of biological chemistry,
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
June 1991,
The Journal of biological chemistry,
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
December 2017,
Journal of thrombosis and haemostasis : JTH,
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
January 2002,
European journal of biochemistry,
J Mimuro, and
S Muramatsu, and
Y Kurano, and
Y Uchida, and
H Ikadai, and
S Watanabe, and
Y Sakata
March 2009,
The international journal of biochemistry & cell biology,
