Biomaterial Database

Neurochemical development of the raphe after continuous prenatal cocaine exposure. 1993

E M Factor, and R P Hart, and G M Jonakait

Department of Biological Sciences, Rutgers University, Newark, NJ 07102.

Disorders among cocaine-exposed infants suggest the medullary raphe nuclei (MRN) as potential targets for cocaine-induced disabilities. Serotonin (5-HT) and substance P (SP) are colocalized within neurons of the MRN. To determine possible neurochemical abnormalities resulting from prenatal cocaine exposure, we measured levels of mRNA coding for the SP preprohormone preprotachykinin (PPT), SP peptide levels, and tryptophan hydroxylase (TPH) activity throughout perinatal and early postnatal development. Pregnant rats at embryonic day 7 (E7) were implanted (SC) with Alzet osmotic minipumps dispensing 10 or 40 mg/kg cocaine daily for 2 weeks. Maternal weight gain, duration of pregnancy, and fetal viability were unaffected by the treatment. Moreover, TPH activity and levels of PPT mRNA [assessed from day E17 through postnatal day (PND) 14] were normal in pups receiving either dose. Except for a transient decline at PND1, SP peptide levels in the ventral spinal cord in the first postnatal week were also unchanged. These data suggest that continuous exposure throughout gestation to these concentrations of cocaine has negligible consequences for the development of these neurotransmitters.

UI	MeSH Term	Description	Entries
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011297	Prenatal Exposure Delayed Effects	The consequences of exposing the FETUS in utero to certain factors, such as NUTRITION PHYSIOLOGICAL PHENOMENA; PHYSIOLOGICAL STRESS; DRUGS; RADIATION; and other physical or chemical factors. These consequences are observed later in the offspring after BIRTH.	Delayed Effects, Prenatal Exposure,Late Effects, Prenatal Exposure
D011498	Protein Precursors		Precursors, Protein
D011903	Raphe Nuclei	Collections of small neurons centrally scattered among many fibers from the level of the TROCHLEAR NUCLEUS in the midbrain to the hypoglossal area in the MEDULLA OBLONGATA.	Caudal Linear Nucleus of the Raphe,Interfascicular Nucleus,Nucleus Incertus,Rostral Linear Nucleus of Raphe,Rostral Linear Nucleus of the Raphe,Superior Central Nucleus,Central Nucleus, Superior,Incertus, Nucleus,Nuclei, Raphe,Nucleus, Interfascicular,Nucleus, Raphe,Nucleus, Superior Central,Raphe Nucleus
D003042	Cocaine	An alkaloid ester extracted from the leaves of plants including coca. It is a local anesthetic and vasoconstrictor and is clinically used for that purpose, particularly in the eye, ear, nose, and throat. It also has powerful central nervous system effects similar to the amphetamines and is a drug of abuse. Cocaine, like amphetamines, acts by multiple mechanisms on brain catecholaminergic neurons; the mechanism of its reinforcing effects is thought to involve inhibition of dopamine uptake.	Cocaine HCl,Cocaine Hydrochloride,HCl, Cocaine,Hydrochloride, Cocaine
D005260	Female		Females
D005314	Embryonic and Fetal Development	Morphological and physiological development of EMBRYOS or FETUSES.	Embryo and Fetal Development,Prenatal Programming,Programming, Prenatal
D005333	Fetus	The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.	Fetal Structures,Fetal Tissue,Fetuses,Mummified Fetus,Retained Fetus,Fetal Structure,Fetal Tissues,Fetus, Mummified,Fetus, Retained,Structure, Fetal,Structures, Fetal,Tissue, Fetal,Tissues, Fetal
D000375	Aging	The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.	Senescence,Aging, Biological,Biological Aging
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia