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Serum prostate-specific antigen (PA) values detected by a newly developed enzyme immunoassay (EIA, MARKIT-M PA) as a successor of MARKIT-F PA, which has been a leading kit in Japan, were evaluated for its role in the diagnosis of cancer of the prostate and follow-up of the patients afflicted with the disease. The system is one-step sandwich type EIA using horseradish peroxidase as a tracer and has 0.50-100 ng/ml of detectable range with small amount of sample volume (25 microliters) and reliable quality control data. Furthermore, serum PA values detected by the assay were almost equivocal to those detected by MARKIT-F PA. Serum PA values in prostate cancer patients (n = 122) were statistically higher than those in normal males (n = 90), urological malignancies other than prostate cancer (n = 48) or benign prostatic hypertrophy (BPH, n = 73). Even in the patients with stage A and B prostate cancer, serum PA values were observed to be statistically higher than those in BPH cases. If 3.6 ng/ml was used, which is normal value in MARKIT-F PA, as a cut-off value and BPH cases as a control, the sensitivity, specificity and efficacy for diagnosis of prostate cancer were 77.9, 91.8 and 83.1%, respectively, which showed the best results during the range examined. Serially determined serum PA values in following up the patients with prostate cancer were confirmed to be highly effective to evaluate treatment responses. These results suggest that MARKIT-M PA is thought to be one of the best tool for determination of serum PA values.
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
October 1992,
Hinyokika kiyo. Acta urologica Japonica,
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
June 1995,
Hinyokika kiyo. Acta urologica Japonica,
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
October 1982,
Horumon to rinsho. Clinical endocrinology,
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
October 1984,
Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine,
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
November 2010,
Talanta,
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
May 1981,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
January 1986,
Rinsho byori. The Japanese journal of clinical pathology,
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
January 1985,
Annals of clinical and laboratory science,
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
October 2002,
Peptides,
M Kuriyama, and
N Esaki, and
I Shinoda, and
S Ito, and
S Yamada, and
K Tokuyama, and
T Deguchi, and
Y Takahashi, and
Y Kawada, and
S Akimoto
May 1992,
Nihon Sanka Fujinka Gakkai zasshi,
