-transparent.png){kind=logo}
NADPH-diaphorase (NADPHd) is known to be identical to nitric oxide (NO) synthase in the mammalian nervous system, and is therefore used as a marker of NO-producing neurones. Using the histochemical reaction for NADPHd, we searched for such neurones in a selection of invertebrates. Special emphasis was given to molluscs. No selective neuronal staining was found in representatives of coelenterates, turbellarians, nematodes and urochordates. In all annelids, arthropods and molluscs examined, with the exception of a chiton, specific neurones were selectively stained. The reaction was particularly strong in pulmonate molluscs where scattered positive neurones were found in various ganglia and clustered symmetrically in the paired buccal ganglia. Biochemical assay of NO synthase in osphradia of the gastropod mollusc Lymnaea stagnalis revealed a formation of citrullin that was inhibited by the specific NO synthase N omega-nitro-L-arginine (NO2Arg). Both histochemical and biochemical methods indicate that NO can be used as a signal molecule by specific neurones in advanced invertebrates.
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
April 2010,
American journal of physiology. Regulatory, integrative and comparative physiology,
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
February 2002,
Hypertension (Dallas, Tex. : 1979),
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
February 2005,
Nitric oxide : biology and chemistry,
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
March 1994,
Infection and immunity,
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
November 2009,
British journal of pharmacology,
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
September 1995,
Nature,
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
August 2017,
Proceedings of the National Academy of Sciences of the United States of America,
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
May 1996,
Brain research,
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
April 2005,
Critical care medicine,
R Elofsson, and
M Carlberg, and
L Moroz, and
L Nezlin, and
D Sakharov
January 2000,
Kokuritsu Iyakuhin Shokuhin Eisei Kenkyujo hokoku = Bulletin of National Institute of Health Sciences,
