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The cytochalasin-B micronucleus/kinetochore assay in vitro: studies with 10 suspected aneugens. 1993

A M Lynch, and J M Parry
Department of Clinical Pharmacology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.

An in vitro micronucleus assay in low passage Chinese hamster Luc2 cells capable of detecting numerical and structural chromosome changes was developed. Chromosome loss was inferred by indirect visualisation of human CREST antikinetochore antibodies bound to centromeres in chemically-induced micronuclei of cytochalasin-B arrested binucleated cells. The assay was used to evaluate 10 chemicals which had been selected for their known or suspected effects upon various components of the cell-division apparatus. These chemicals were colchicine (COL), vinblastine (VBL), thiabendazole (TBZ), chloral hydrate (CH), thimerosal (TM), diazepam (DZ), pyrimethamine (PYR), hydroquinone (HQ), cadmium chloride (CdCl2) and econazole nitrate (EZ). Mitomycin-C (MMC) was used as a positive control for the induction of micronuclei. 8 of the core chemicals induced micronuclei in Chinese hamster Luc2 cells. 4 of the chemicals (COL, VBL, TBZ, CH) increased levels of micronuclei which were positive for kinetochore antibody labelling and hence chromosome loss. 3 of the chemicals (DZ, PYR, HQ) and the positive control (MMC) increased the levels of Mn which were negative for kinetochore antibody labelling. The results with TM were equivocal and EN was negative. The results of these studies suggest that the cytochalasin-B Mn/k assay is a cost-effective, simple and rapid alternative to classical cytogenetic assays for the detection of chemically induced aneuploidy.

UI MeSH Term Description Entries
D009153 Mutagens Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes. Clastogen,Clastogens,Genotoxin,Genotoxins,Mutagen
D011739 Pyrimethamine One of the FOLIC ACID ANTAGONISTS that is used as an antimalarial or with a sulfonamide to treat toxoplasmosis. Chloridin,Daraprim,Malocide,Tindurine
D002104 Cadmium An element with atomic symbol Cd, atomic number 48, and atomic weight 112.41. It is a metal and ingestion will lead to CADMIUM POISONING.
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002503 Centromere The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division. Centromeres
D002697 Chloral Hydrate A hypnotic and sedative used in the treatment of INSOMNIA. Noctec,Hydrate, Chloral
D002712 Chlorides Inorganic compounds derived from hydrochloric acid that contain the Cl- ion. Chloride,Chloride Ion Level,Ion Level, Chloride,Level, Chloride Ion
D003078 Colchicine A major alkaloid from Colchicum autumnale L. and found also in other Colchicum species. Its primary therapeutic use is in the treatment of gout, but it has been used also in the therapy of familial Mediterranean fever (PERIODIC DISEASE). Colchicine, (+-)-Isomer,Colchicine, (R)-Isomer
D003412 Cricetulus A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research. Hamsters, Armenian,Hamsters, Chinese,Hamsters, Grey,Armenian Hamster,Armenian Hamsters,Chinese Hamster,Chinese Hamsters,Grey Hamster,Grey Hamsters,Hamster, Armenian,Hamster, Chinese,Hamster, Grey

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