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A dominant negative Raf-1 mutant prevents v-Src-induced transformation. 1993
S A Qureshi, and
C K Joseph, and
M Hendrickson, and
J Song, and
R Gupta, and
J Bruder, and
U Rapp, and
D A Foster
Institute for Biomolecular Structure and Function, Hunter College, City University of New York, New York 10021.
A vector expressing a dominant negative mutant of Raf-1 was stably introduced into BALB/c 3T3 cells expressing a temperature-sensitive derivative of v-Src. High levels of the Raf-mutant were detected in these cells. Expression of the Raf-1 mutant blocked v-Src-induced transformation, as determined by reversion to a flat non-transformed morphology and the inability to form colonies in soft agar. Cells transfected with the parental vector lacking the mutant Raf-1 could be transformed by v-Src. These data suggest that intracellular signals activated by v-Src that are mediated by Raf-1 are required for transformation by v-Src.
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