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The genomic localization of two immunodominant genes encoding two proteins of the Epstein-Barr virus capsid antigen (VCA) complex, VCA-p18 and VCA-p40, has been identified. For that purpose, lambda gt11-based cDNA libraries were constructed from HH514.c16 cells induced for virus production. The libraries were screened with a monoclonal antibody, EBV.OT41A, directed against VCA-p40 or with affinity-purified human antibodies against VCA-p18. Sequencing of the inserts of positive plaques showed that VCA-p18 and VCA-p40 are encoded within open reading frames (ORFs) BFRF3 and BdRF1, respectively. Peptide scanning analysis of the predicted protein of ORF BdRF1 resulted in defining the epitope of monoclonal antibody EBV.OT41A at the C-terminal region. The dominant VCA-p18 reactivity of human sera can be completely inhibited by preadsorption with Escherichia coli-expressed BFRF3-beta-galactosidase. Serum of a rabbit immunized with BFRF3-beta galactosidase reacts with a VCA-specific protein of 18 kDa. In addition, BFRF3-beta-galactosidase affinity-purified antibodies react with VCA-p18 of virus-producing cells (HH514.c16). Complete inhibition of viral DNA polymerase activity by phosphonoacetic acid is associated with the absence of RNAs and protein products of both ORFs, indicating that VCA-p18 and VCA-p40 are true late antigens.
W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
February 2011,
Journal of virology,
W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
February 2009,
The Journal of general virology,
W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
February 1989,
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W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
October 1992,
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W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
January 1987,
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W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
September 1991,
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W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
September 2001,
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W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
September 1992,
Casopis lekaru ceskych,
W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
January 1991,
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W M van Grunsven, and
E C van Heerde, and
H J de Haard, and
W J Spaan, and
J M Middeldorp
May 1988,
The Journal of general virology,
