New staining methods for semithin sections of tissues of Seriola dumerili embedded in methacrylate. 1993

M C Sarasquete, and M L Gonzalez de Canales
Instituto de Ciencias Marinas de Andalucía, Cádiz, Spain.

Semithin sections of different tissues of Seriola dumerili R. Embedded in glycol methacrylate, were stained with two staining methods normally used in conventional haematology and histology: Gutierrez's cytopanchrome and Gutierrez's polychrome V.O.F. The results observed in all tissues embedded in this resin and stained with V.O.F. are satisfactory and comparable, as to staining affinity, to those obtained on paraffin-embedded tissues. Gutierrez's trichrome stain appears to be a useful tool in the study of oogenesis for detecting changes in staining according to cellular structure and maturation stage. The results observed with cytopanchrome are similar to those obtained with toluidine blue in glycol methacrylate-embedded tissues. The use of these two staining techniques, requires no previous oxidation, alkali treatments, high temperatures or removal of the embedding medium. Furthermore, both dyes can be used at the same time: in this case, cytoplasm of previtellogenic oocytes showed a stronger affinity for cytopanchrome, and were more intensely stained by V.O.F. as the vitellogenesis of Seriola dumerili progressed. Both stain, and especially the cytopanchrome one, also are available for staining the liver, gills and testis.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D008689 Methacrylates Acrylic acids or acrylates which are substituted in the C-2 position with a methyl group. Methacrylate
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D009866 Oogenesis The process of germ cell development in the female from the primordial germ cells through OOGONIA to the mature haploid ova (OVUM). Oogeneses
D010053 Ovary The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE. Ovaries
D003593 Cytoplasm The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Protoplasm,Cytoplasms,Protoplasms
D005260 Female Females
D005399 Fishes A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D005880 Gills Paired respiratory organs of fishes and some amphibians that are analogous to lungs. They are richly supplied with blood vessels by which oxygen and carbon dioxide are exchanged directly with the environment. Gill

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