BIOMAT Database Logo BIOMAT Database Logo

Comparison of interactions of 5'-derivatives of deoxyoctathymidylate with human DNA polymerize alpha and HIV reverse transcriptase. 1993

S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk.

Km and Vmax values for d(pT8) and its derivatives containing various 5'-end groups were estimated in the reaction of DNA polymerization alpha catalyzed by DNA polymerase alpha and HIV-RT. The effect of 5'-end modification of primer is more pronounced in the case of HIV-RT. Strong influence is observed for an intercalating (ethidium) group. The affinity of EtpT8 is 200-fold higher than that of d(pT8). Attachment of Phn-, Dnm- and Hem-groups results in the increase of affinity of modified primer from 10 up to 20 times. For DNA polymerase alpha the influence of modifiers on primer affinity is much weaker. The effect of 5'-end residues on the Vmax values is also more pronounced for HIV RT. The way to improve selective interaction of oligonucleotide derivatives with the primer site of HIV RT is suggested.

UI MeSH Term Description Entries
D011071 Poly T A group of thymine nucleotides in which the phosphate residues of each thymine nucleotide act as bridges in forming diester linkages between the deoxyribose moieties. Poly dT,Polythymidylic Acids,Thymine Polynucleotides,Polydeoxythymidylate,Acids, Polythymidylic,Polynucleotides, Thymine,dT, Poly
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004257 DNA Polymerase II A DNA-dependent DNA polymerase characterized in E. coli and other lower organisms. It may be present in higher organisms and has an intrinsic molecular activity only 5% of that of DNA Polymerase I. This polymerase has 3'-5' exonuclease activity, is effective only on duplex DNA with gaps or single-strand ends of less than 100 nucleotides as template, and is inhibited by sulfhydryl reagents. DNA Polymerase epsilon,DNA-Dependent DNA Polymerase II,DNA Pol II,DNA Dependent DNA Polymerase II
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012194 RNA-Directed DNA Polymerase An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 2.7.7.49. DNA Polymerase, RNA-Directed,RNA-Dependent DNA Polymerase,Reverse Transcriptase,RNA Transcriptase,Revertase,DNA Polymerase, RNA Directed,DNA Polymerase, RNA-Dependent,RNA Dependent DNA Polymerase,RNA Directed DNA Polymerase
D015394 Molecular Structure The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds. Structure, Molecular,Molecular Structures,Structures, Molecular
D015497 HIV-1 The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte. Human immunodeficiency virus 1,HIV-I,Human Immunodeficiency Virus Type 1,Immunodeficiency Virus Type 1, Human
D054303 HIV Reverse Transcriptase A reverse transcriptase encoded by the POL GENE of HIV. It is a heterodimer of 66 kDa and 51 kDa subunits that are derived from a common precursor protein. The heterodimer also includes an RNAse H activity (RIBONUCLEASE H, HUMAN IMMUNODEFICIENCY VIRUS) that plays an essential role the viral replication process. Reverse Transcriptase, HIV,Reverse Transcriptase, Human Immunodeficiency Virus,Transcriptase, HIV Reverse

Related Publications

S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Inhibition of HIV-associated reverse transcriptase by sugar-modified derivatives of thymidine 5'-triphosphate in comparison to cellular DNA polymerases alpha and beta.
October 1987, Biochemical and biophysical research communications,
S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Murine leukemia virus reverse transcriptase: structural comparison with HIV-1 reverse transcriptase.
June 2008, Virus research,
S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Mass Spectrometric Characterization of HIV-1 Reverse Transcriptase Interactions with Non-nucleoside Reverse Transcriptase Inhibitors.
January 2016, Biological & pharmaceutical bulletin,
S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Base mispair extension kinetics. Comparison of DNA polymerase alpha and reverse transcriptase.
February 1990, The Journal of biological chemistry,
S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Fidelity of DNA synthesis catalyzed by human DNA polymerase alpha and HIV-1 reverse transcriptase: effect of reaction pH.
November 1993, Nucleic acids research,
S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Probing interactions between HIV-1 reverse transcriptase and its DNA substrate with backbone-modified nucleotides.
February 1999, Chemistry & biology,
S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Biochemical interactions between HIV-1 integrase and reverse transcriptase.
March 2013, FEBS letters,
S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
HIV-reverse transcriptase and human DNA polymerase alpha share amino acid sequence homologies to bacterial penicillin-binding proteins.
January 1990, Methods and findings in experimental and clinical pharmacology,
S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Differential inhibition of HIV-reverse transcriptase and various DNA and RNA polymerases by some catechin derivatives.
January 1989, Nucleic acids symposium series,
S N Khodyreva, and V N Podust, and D S Sergeev, and E M Ivanova, and E I Frolova, and A A Koshkin, and T S Godovikova, and V F Zarytova, and M Ricchetti, and O I Lavrik
Combinative interactions of a human immunodeficiency virus (HIV) Tat antagonist with HIV reverse transcriptase inhibitors and an HIV protease inhibitor.
February 1994, Antimicrobial agents and chemotherapy,
Copied contents to your clipboard!