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Cryopreservation techniques have become essential in longitudinal evaluation of lymphocyte function. This study describes a convenient method for freezing of peripheral blood mononuclear cells, using a small cryocontainer. Analysis of cell function, assayed by lymphoproliferation to recall antigens and by cytotoxic capacity of activated lymphocytes, was performed in parallel on fresh and frozen-thawed cells. Although cryopreservation did affect lymphocyte function, our results indicate that this freezing method performed equally well compared to a computer controlled device. We conclude that the cryocontainer has proved to be a suitable and practical tool in clinical studies and is an economical alternative to conventional methods.
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
January 2012,
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference,
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
April 1982,
Analytical biochemistry,
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
February 1972,
Journal of medicinal chemistry,
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
August 1970,
Analytical biochemistry,
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
January 2016,
Methods in molecular biology (Clifton, N.J.),
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
January 2023,
Talanta,
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
July 2016,
Journal of clinical oncology : official journal of the American Society of Clinical Oncology,
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
July 2020,
The Journal of urology,
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
July 2007,
Analytical biochemistry,
J Vingerhoets, and
G Vanham, and
L Kestens, and
P Gigase
April 1990,
BioTechniques,
