The disynaptic pathway from muscle spindle Ia afferents to motoneurones of the antagonist muscle is one of the best studied pathways in the spinal cord. Early animal studies--mainly in the cat--have provided a detailed knowledge of the pathway itself and of the integration of segmental and supraspinal convergence at the interneuronal level. Although this knowledge was used to formulate hypotheses on the function of the pathway during natural movements, the reduced animal preparation limited the possibilities of testing these ideas. However, such information has more recently been obtained from human subjects by using indirect electrophysiological techniques. In most of these experiments the disynaptic Ia inhibition was demonstrated as a short-latency depression of a monosynaptic test reflex (H-reflex) following a conditioning stimulation of the antagonist nerve. Changes in the size of this depression during voluntary tasks were then taken as evidence of a central regulation of the pathway. It has for example been demonstrated in this way that the brain regulates the Ia inhibitory interneurones in parallel with their corresponding motoneurones during extension-flexion movements, but not during co-contraction of antagonistic muscles. The importance of the central control of the pathway has also been emphasized by the finding of a disordered regulation of its activity in patients with lesions of the brain. This may possibly contribute to the inappropriate co-contraction of antagonistic muscles observed in some of these patients. It seems reasonable to expect that this kind of experiment in the future may contribute significantly to the knowledge of the central control of spinal motor mechanisms.