-transparent.png){kind=logo}
We previously described the only satisfactory procedure yet achieved for separating uric acid and allantoin from rat liver. The procedure was based on trichloroacetic acid (TCA) extraction, acid hydrolysis, treatment with Hg-acetate, and cation- and anion-exchange chromatography. After separation, allantoin was quantified by a colorimetric method, and uric acid enzymatically using uricase. Since this procedure is too time-consuming, we propose an improved version which avoids the need for anion-exchange chromatography and the complex assay of catabolic compounds. The new method consists of a very fast and simple HPLC separation and direct determination of uric acid and allantoin at 220 nm. The method can be used for fresh tissue or after treatment of the tissue with labeled precursor.
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
July 1992,
Biochimica et biophysica acta,
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
January 1998,
Advances in experimental medicine and biology,
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
July 1953,
Bollettino della Societa italiana di biologia sperimentale,
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
January 1991,
Advances in experimental medicine and biology,
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
November 1992,
Biochemical Society transactions,
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
April 1950,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
February 2007,
Electrophoresis,
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
November 1970,
Veterinary medicine, small animal clinician : VM, SAC,
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
February 2023,
Journal of pharmaceutical and biomedical analysis,
L Terzuoli, and
M Pandolfi, and
L Arezzini, and
M Pizzichini, and
E Marinello, and
R Pagani
October 1963,
Archivio per le scienze mediche,
