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We examined effect of insulin or 12-O-tetradecanoyl phorbol 13-acetate (TPA) on the subcellular redistribution of protein kinase C isoforms in rat adipocytes. Total Mono Q column-elutable novel PKCs (nPKCs) which are Ca(2+)-independent and phospholipid-dependent protein kinases, decreased in the cytosolic fraction and increased in the membrane fraction during treatment with insulin or phorbol ester for 10 min. Immunoblot analysis of novel PKCs, -epsilon, -delta and -zeta, showed that insulin stimulated the translocation of these PKC isoforms from cytosol to membrane, similar to the translocation of conventional Ca2+/phospholipid-dependent PKCs (cPKCs), -alpha, -beta, and -gamma. Phorbol esters stimulated the translocation of PKC-alpha, -beta, -gamma, -epsilon and -delta, but not PKC-zeta. These results suggest that (a) insulin and phorbol esters similarly stimulate the translocation of each PKC isoform except for PKC-zeta, and (b) the translocation of both nPKCs and cPKCs occurs during insulin and TPA actions in rat adipocytes.
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
November 1992,
The Biochemical journal,
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
March 1986,
Biochemical and biophysical research communications,
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
January 2006,
Journal of neurochemistry,
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
December 1999,
Molecular and cellular biochemistry,
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
September 2002,
The international journal of biochemistry & cell biology,
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
January 1993,
Cytobios,
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
May 1995,
Biochimica et biophysica acta,
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
October 1991,
Diabetes,
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
January 2014,
European journal of pharmacology,
T Ishizuka, and
M Yamamoto, and
K Kajita, and
T Nagashima, and
O Taniguchi, and
H Wada, and
S Itaya, and
K Yasuda
September 2007,
Biochimica et biophysica acta,
