Biomaterial Database

Differential effects of spinalization on discharge patterns and discharge rates of simultaneously recorded nociceptive and non-nociceptive spinal dorsal horn neurons. 1995

J Sandkühler, and A Eblen-Zajjur, and Q-G Fu, and C Forster

II. Physiologisches Institut, Universität Heidelberg, 69120 Heidelberg Germany Institut für Physiologie und Biokybernetik, Universität Erlangen, 91054 Erlangen Germany.

Recordings were made simultaneously from 2-5 neurons at the same site in the lumbar spinal dorsal horn of pentobarbital-anesthetized rats. Neurons were classified as low-threshold (LT) or multireceptive (MR) according to their responses to non-noxious mechanical or noxious radiant heat stimuli of the skin. At the same recording sites neurons could be encountered which belong to different classes and/or which had mechanoreceptive fields which did not overlap. Cold blocks of the upper or lower thoracic cord or transsections of the upper cervical cord were made to evaluate the effects of spinalization on both the rate and pattern of background activity and/or noxious heat-evoked responses of different dorsal horn neurons under identical experimental conditions. At 24 of 27 recording sites, spinalization had qualitatively or quantitatively different effects on the rate of background activity of simultaneously recorded neurons. Interspike interval (ISI) means of background activity were significantly reduced in 29 of 65 (44.6%) neurons, prolonged in 23 of 65 (35.4%) neurons, or unchanged in 13 of 65 (20%) neurons. MR neurons displayed a significantly higher incidence of decreased background activity 17 of 45 (37.8%) and a lower incidence of increased background activity (18 of 45, 40%) during spinalization than the LT neurons from which 1 of 12 (8.3%) decreased and 8 of 12 (66.6%) increased background activity. Almost all (95.4%) neurons changed their discharge patterns after spinalization. At 9 of 27 recording sites, the discharge patterns of simultaneously recorded neurons were affected differently by spinalization as revealed by the coefficient of dispersion of the interspike intervals (ISI), indicating changes in the tendency to discharge action potential in clusters (bursts). At the same recording sites the level of noxious heat-evoked responses of simultaneously recorded MR neurons was also differentially affected by spinalization. Nociceptive responses were significantly enhanced in 19 of 37 (51.4%) neurons (137.8 +/- 142.6% of control, mean +/- SD), reduced in 13 of 37 neurons (35.1%) (by 58.9 +/- 20.9%) and/or unchanged in 5 of 37 (13.5%) neurons. It is concluded that no general 'tone' of descending antinociception exists and that tonic descending excitatory and inhibitory systems may be active simultaneously modulating both the level and pattern of neuronal discharges.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009474	Neurons	The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.	Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D009619	Nociceptors	Peripheral AFFERENT NEURONS which are sensitive to injuries or pain, usually caused by extreme thermal exposures, mechanical forces, or other noxious stimuli. Their cell bodies reside in the DORSAL ROOT GANGLIA. Their peripheral terminals (NERVE ENDINGS) innervate target tissues and transduce noxious stimuli via axons to the CENTRAL NERVOUS SYSTEM.	Pain Receptors,Receptors, Pain,Nociceptive Neurons,Neuron, Nociceptive,Neurons, Nociceptive,Nociceptive Neuron,Nociceptor,Pain Receptor
D001794	Blood Pressure	PRESSURE of the BLOOD on the ARTERIES and other BLOOD VESSELS.	Systolic Pressure,Diastolic Pressure,Pulse Pressure,Pressure, Blood,Pressure, Diastolic,Pressure, Pulse,Pressure, Systolic,Pressures, Systolic
D003080	Cold Temperature	An absence of warmth or heat or a temperature notably below an accustomed norm.	Cold,Cold Temperatures,Temperature, Cold,Temperatures, Cold
D005071	Evoked Potentials	Electrical responses recorded from nerve, muscle, SENSORY RECEPTOR, or area of the CENTRAL NERVOUS SYSTEM following stimulation. They range from less than a microvolt to several microvolts. The evoked potential can be auditory (EVOKED POTENTIALS, AUDITORY), somatosensory (EVOKED POTENTIALS, SOMATOSENSORY), visual (EVOKED POTENTIALS, VISUAL), or motor (EVOKED POTENTIALS, MOTOR), or other modalities that have been reported.	Event Related Potential,Event-Related Potentials,Evoked Potential,N100 Evoked Potential,P50 Evoked Potential,N1 Wave,N100 Evoked Potentials,N2 Wave,N200 Evoked Potentials,N3 Wave,N300 Evoked Potentials,N4 Wave,N400 Evoked Potentials,P2 Wave,P200 Evoked Potentials,P50 Evoked Potentials,P50 Wave,P600 Evoked Potentials,Potentials, Event-Related,Event Related Potentials,Event-Related Potential,Evoked Potential, N100,Evoked Potential, N200,Evoked Potential, N300,Evoked Potential, N400,Evoked Potential, P200,Evoked Potential, P50,Evoked Potential, P600,Evoked Potentials, N100,Evoked Potentials, N200,Evoked Potentials, N300,Evoked Potentials, N400,Evoked Potentials, P200,Evoked Potentials, P50,Evoked Potentials, P600,N1 Waves,N2 Waves,N200 Evoked Potential,N3 Waves,N300 Evoked Potential,N4 Waves,N400 Evoked Potential,P2 Waves,P200 Evoked Potential,P50 Waves,P600 Evoked Potential,Potential, Event Related,Potential, Event-Related,Potential, Evoked,Potentials, Event Related,Potentials, Evoked,Potentials, N400 Evoked,Related Potential, Event,Related Potentials, Event,Wave, N1,Wave, N2,Wave, N3,Wave, N4,Wave, P2,Wave, P50,Waves, N1,Waves, N2,Waves, N3,Waves, N4,Waves, P2,Waves, P50
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013116	Spinal Cord	A cylindrical column of tissue that lies within the vertebral canal. It is composed of WHITE MATTER and GRAY MATTER.	Coccygeal Cord,Conus Medullaris,Conus Terminalis,Lumbar Cord,Medulla Spinalis,Myelon,Sacral Cord,Thoracic Cord,Coccygeal Cords,Conus Medullari,Conus Terminali,Cord, Coccygeal,Cord, Lumbar,Cord, Sacral,Cord, Spinal,Cord, Thoracic,Cords, Coccygeal,Cords, Lumbar,Cords, Sacral,Cords, Spinal,Cords, Thoracic,Lumbar Cords,Medulla Spinali,Medullari, Conus,Medullaris, Conus,Myelons,Sacral Cords,Spinal Cords,Spinali, Medulla,Spinalis, Medulla,Terminali, Conus,Terminalis, Conus,Thoracic Cords
D013126	Spinal Nerve Roots	Paired bundles of NERVE FIBERS entering and leaving the SPINAL CORD at each segment. The dorsal and ventral nerve roots join to form the mixed segmental spinal nerves. The dorsal roots are generally afferent, formed by the central projections of the spinal (dorsal root) ganglia sensory cells, and the ventral roots are efferent, comprising the axons of spinal motor and PREGANGLIONIC AUTONOMIC FIBERS.	Dorsal Roots,Spinal Roots,Ventral Roots,Dorsal Root,Nerve Root, Spinal,Nerve Roots, Spinal,Root, Dorsal,Root, Spinal,Root, Spinal Nerve,Root, Ventral,Roots, Dorsal,Roots, Spinal,Roots, Spinal Nerve,Roots, Ventral,Spinal Nerve Root,Spinal Root,Ventral Root
D017207	Rats, Sprague-Dawley	A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.	Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats