Biomaterial Database

[Purification and characteristics of AMP-deaminase from trout white muscle]. 1995

V I Lushchak, and K B Storey

AMP-deaminase was purified from trout white muscle and some of its properties were investigated. The enzyme preparation was electrophoretically homogeneous; the molecular mass of the polypeptides was equal to 71600 +/- 550 Da, the specific activity was 200-500 U./mg of protein. Activation of the enzyme caused by acidification of the medium in the physiological range of pH was the result of reduction of Km for the substrate. ADP and ATP activated the enzyme, while GTP inhibited it. The enzyme was also inhibited by IMP (this phenomenon had never been described before). A change in pH within the physiological range of pH (6.6-7.3) had no influence on ATP, GTP or IMP effects on AMP-deaminase. The enzyme activation by ADP was sensitive to pH. The possibilities of fish muscle AMP-deaminase regulation under conditions of intensified metabolism is discussed.

UI	MeSH Term	Description	Entries
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009711	Nucleotides	The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)	Nucleotide
D004789	Enzyme Activation	Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.	Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000659	AMP Deaminase	An enzyme that catalyzes the deamination of AMP to IMP. EC 3.5.4.6.	AMP Aminase,Adenylate Deaminase,5'-AMP Deaminase,AMP Aminohydrolase,Myoadenylate Deaminase,5' AMP Deaminase,Aminase, AMP,Aminohydrolase, AMP,Deaminase, 5'-AMP,Deaminase, AMP,Deaminase, Adenylate,Deaminase, Myoadenylate
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D014337	Trout	Various fish of the family SALMONIDAE, usually smaller than salmon. They are mostly restricted to cool clear freshwater. Some are anadromous. They are highly regarded for their handsome colors, rich well-flavored flesh, and gameness as an angling fish. The genera Salvelinus, Salmo, and ONCORHYNCHUS have been introduced virtually throughout the world.	Chars,Salvelinus,Char