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This paper recommends a simple and easy method to isolate and purify glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Schistosoma japonicum with electrophoretic purity. The recovery rate of 56.1% of glyceraldehyde-3-phosphate dehydrogenase from S. japonicum extract was achieved by ammonium sulfate precipitation, DEAE-cellulose column chromatography and SDS-PAGE. Using SDS-PAGE, the presence of one major band in the final purified GAPDH preparation was demonstrated. This protein had a MW of 37 kDa. The authors discuss the important functions of GAPDH and suggest that this method may be useful in the research of the protective immunity of S. japonicum GAPDH in the development of schistosome vaccine.
Q Wang, and
M Wang, and
H Chang
November 2022,
Acta tropica,
Q Wang, and
M Wang, and
H Chang
February 2020,
Acta tropica,
Q Wang, and
M Wang, and
H Chang
May 2009,
Acta biochimica et biophysica Sinica,
Q Wang, and
M Wang, and
H Chang
January 1994,
Antonie van Leeuwenhoek,
Q Wang, and
M Wang, and
H Chang
January 1988,
Acta biochimica Polonica,
Q Wang, and
M Wang, and
H Chang
November 2016,
Protein expression and purification,
Q Wang, and
M Wang, and
H Chang
January 1968,
Enzymologia biologica et clinica,
Q Wang, and
M Wang, and
H Chang
January 1982,
Methods in enzymology,
Q Wang, and
M Wang, and
H Chang
January 1969,
Biokhimiia (Moscow, Russia),
Q Wang, and
M Wang, and
H Chang
December 1979,
Archives of biochemistry and biophysics,
