-transparent.png){kind=logo}
In cultured gastric mucosal cells, we investigated whether: (1) adaptive cytoprotection was associated with stimulation of endogenous prostaglandin synthesis; (2) prostaglandins given exogenously were cytoprotective against ethanol-induced gastric mucosal cell damage; and (3) a relationship existed between cytoprotection and mucus release. Cytolysis was quantified by measuring 51Cr release from prelabeled cells. Mucus release was determined by measurement of [3H]glucosamine release. Concentrations of ethanol > 12% caused cell damage and increased 51Cr release dose dependently. Pretreatment with low concentrations of ethanol (0.5-1.5%) decreased ethanol-induced 51Cr release, but also decreased prostaglandin E2 synthesis. Prostaglandin E2 and 16,16-dimethyl prostaglandin E2 given exogenously were cytoprotective against ethanol-induced gastric mucosal cell damage. Treatment with low concentrations of ethanol (1.5%) increased mucus release from cultured gastric mucosal cells. However, prostaglandin E2 and 16,16-dimethyl prostaglandin E2 did not affect mucus release. We conclude that in cultured gastric mucus-producing cells: (1) adaptive cytoprotection occurs without stimulation of endogenous prostaglandin synthesis but with increase in mucus release; and (2) exogenous prostaglandins are cytoprotective against ethanol-induced gastric mucosal cell damage without stimulating mucus release in vitro. We postulate that adaptive cytoprotection in cultured gastric mucus-producing cells is not mediated by prostaglandin, but by mucus released in response to a mild irritant.
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
January 1988,
Digestion,
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
June 1980,
The American journal of gastroenterology,
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
August 1990,
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology,
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
May 1993,
The American journal of physiology,
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
April 1987,
Sheng li xue bao : [Acta physiologica Sinica],
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
March 1983,
Gastroenterology,
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
January 1990,
Journal of clinical gastroenterology,
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
September 1999,
Inflammation research : official journal of the European Histamine Research Society ... [et al.],
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
February 1985,
The Medical journal of Australia,
H Mutoh, and
S Ota, and
H Hiraishi, and
K J Ivey, and
A Terano, and
T Sugimoto
February 1985,
The Medical journal of Australia,
