Acanthamoeba keratitis is a rare pathology which is currently observed with increasing frequency in France. Two new cases are reported in young patients wearing soft hydrophile contact lenses. One patient apparently followed cleansing instructions, but the other did not. The essential parasitological diagnosis was confirmed rapidly after comeal sampling, but had been highly suspected from the clinical symptomatology. Treatment combining propamidine and eye-drop antibiotics led to recovery in both cases. Anatomical damage was however observed in one case and a penetrating graft was necessary.