BIOMAT Database Logo BIOMAT Database Logo

Effect of growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments. 1995

C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, Pennsylvania, USA.

Growth factors have been shown to stimulate fibroblast division and thus may influence ligament healing. We analyzed the effects of individual growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments of the rabbit in vitro in order to identify growth factors that might enhance proliferation of fibroblasts and to compare the responses of the fibroblasts from the two ligaments to these growth factors. Through measurement of the uptake of [3H]-thymidine into DNA, fibroblasts from these ligaments that had been treated with epidermal growth factor and basic fibroblast growth factor were found to proliferate nearly eight times more than control fibroblasts. Additionally, the fibroblasts of both ligaments proliferated at similar rates when exposed to platelet-derived growth factor-AA, platelet-derived growth factor-BB, basic fibroblast growth factor, insulin-like growth factor-1, and interleukin-1-alpha. However, epidermal growth factor and transforming growth factor-beta caused the fibroblasts from the medial collateral ligament to proliferate at a rate 1.3-1.4 times greater than that of fibroblasts from the anterior cruciate ligament. The reverse was true with acidic fibroblast growth factor, which stimulated the fibroblasts from the anterior cruciate ligament to proliferate at a rate 1.3-1.6 times greater than that of fibroblasts from the medial collateral ligament. This study demonstrated that growth factors can stimulate cell division in ligaments and may be effective in enhancing ligament healing but that these differences were not great enough to explain fully the clinical differences observed between healing of the medial collateral and anterior cruciate ligaments.

UI MeSH Term Description Entries
D008297 Male Males
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D006133 Growth Substances Signal molecules that are involved in the control of cell growth and differentiation. Mitogens, Endogenous,Endogenous Mitogens
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016118 Anterior Cruciate Ligament A strong ligament of the knee that originates from the posteromedial portion of the lateral condyle of the femur, passes anteriorly and inferiorly between the condyles, and attaches to the depression in front of the intercondylar eminence of the tibia. Cruciate Ligament, Anterior,Anterior Cranial Cruciate Ligament,Cranial Cruciate Ligament,Anterior Cruciate Ligaments,Cranial Cruciate Ligaments,Cruciate Ligament, Cranial,Cruciate Ligaments, Anterior,Cruciate Ligaments, Cranial,Ligament, Anterior Cruciate,Ligament, Cranial Cruciate,Ligaments, Anterior Cruciate,Ligaments, Cranial Cruciate
D017888 Medial Collateral Ligament, Knee The ligament that travels from the medial epicondyle of the FEMUR to the medial margin and medial surface of the TIBIA. The medial meniscus is attached to its deep surface. Collateral Ligament, Medial, Knee,Knee Medial Collateral Ligament,Ligamentum Collaterale Tibiale,Medial Ligament of Knee,Tibial Collateral Ligament,Collateral Ligament, Tibial,Collateral Ligaments, Tibial,Collaterale Tibiale, Ligamentum,Collaterale Tibiales, Ligamentum,Knee Medial Ligament,Knee Medial Ligaments,Ligament, Tibial Collateral,Ligaments, Tibial Collateral,Ligamentum Collaterale Tibiales,Tibial Collateral Ligaments,Tibiale, Ligamentum Collaterale,Tibiales, Ligamentum Collaterale

Related Publications

C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
Synergistic effect of growth factors on cell outgrowth from explants of rabbit anterior cruciate and medial collateral ligaments.
May 1995, Journal of orthopaedic research : official publication of the Orthopaedic Research Society,
C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
Growth factor expression in healing rabbit medial collateral and anterior cruciate ligaments.
January 1998, The Iowa orthopaedic journal,
C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
[The effect of fibroblast growth factors and epidermal growth factors on the proliferation of rabbit anterior cruciate and medial collateral ligament cell in vitro].
March 2005, Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery,
C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
Management of combined injuries of the anterior cruciate and medial collateral ligaments.
January 1996, Instructional course lectures,
C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
Treatment of combined complete tears of the anterior cruciate and medial collateral ligaments.
January 2012, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association,
C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
The effect of anterior cruciate ligament deficiency on the in vivo elongation of the medial and lateral collateral ligaments.
February 2007, The American journal of sports medicine,
C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
Primary reconstruction of the medial collateral ligament in combined injury of the medial collateral and anterior cruciate ligaments. Short-term results.
January 1998, Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA,
C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
Establishment and comparison of fibroblast cell lines from the medial collateral and anterior cruciate ligaments of the rabbit.
June 1990, In vitro cellular & developmental biology : journal of the Tissue Culture Association,
C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
Ultrastructural morphometry of anterior cruciate and medial collateral ligaments: an experimental study in rabbits.
January 1992, Journal of orthopaedic research : official publication of the Orthopaedic Research Society,
C C Schmidt, and H I Georgescu, and C K Kwoh, and G L Blomstrom, and C P Engle, and L A Larkin, and C H Evans, and S L Woo
Analysis of skiing accidents involving combined injuries to the medial collateral and anterior cruciate ligaments.
January 1997, The American journal of sports medicine,
Copied contents to your clipboard!