Biomaterial Database

Reliability criteria for steroid radioimmunoassay. 1975

G E Abraham

The principle of radioimmunoassay has been applied to every hormonal steroid and many steroids without known biological activity. The validation of steroid radioimmunoassay requires the evaluation of reliability criteria. These criteria involve the degree of relative specificity of the assay, the sensitivity which dictates the volume of biological fluid required for accurate and precise measurement, and the precision and accuracy of the assay which should be evaluated in every batch of assays as quality control. The use of steroid radioimmunoassay in physiological and clinical studies requires prior validation of these assays by proper evaluation of the reliability criteria.

UI	MeSH Term	Description	Entries
D011786	Quality Control	A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)	Control, Quality,Controls, Quality,Quality Controls
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D013256	Steroids	A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain vitamins, bile acids, alcohols (STEROLS), and certain natural drugs and poisons. Steroids have a common nucleus, a fused, reduced 17-carbon atom ring system, cyclopentanoperhydrophenanthrene. Most steroids also have two methyl groups and an aliphatic side-chain attached to the nucleus. (From Hawley's Condensed Chemical Dictionary, 11th ed)	Steroid,Catatoxic Steroids,Steroids, Catatoxic