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In asthmatic children without he egg allergy, spontaneous IgE production of PBMC showed 1.27 +/- 0.28 IU/ml (Mean +/- SE) and IgE production of Dermatophagoides farinae (Df) stimulated PBMC after which IFN-gamma decreased to 0.89 +/- 0.23 IU/ml (Mean +/- SE) (p < 0.001). IgE production of Ovalbumin (OVA) stimulated PBMC after which IFN-gamma showed no decrease. In asthmatic children with hen egg allergy, IgE production of OVA stimulated PBMC and IFN-gamma decreased to 1.0 +/- 0.52 IU/ml (Mean +/- SE). In spontaneous IgE production it was 1.61 +/- 0.91 IU/ml. (p < 0.05). IL-4 mRNA was expressed in spontaneous PBMC in asthmatic children, but not in healthy non atopic children. The expression of IL-4 mRNA was suppressed in Df stimulated PBMC followed by IFN-gamma in asthmatic children. In asthmatic children, IFN-gamma mRNA were expressed in Df stimulated PBMC after which IFN-gamma. And simultaneously IgE production in Df stimulated PBMC were suppressed by addition of IFN-gamma.
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