BIOMAT Database Logo BIOMAT Database Logo

Elimination of ingested 32P-labelled E. coli from rat polymorphonuclear neutrophils (PMN). Evaluation of a method. 1976

T Midtvedt, and A Baardsen

A method to be used for in vitro assay of the elimination of ingested 32P-labelled E. coli from rat PMN monolayers has been evaluated. The rate of expulsion of bacterial label from phagocytes into the extracellular medium was found to range between 40 and 50% of the total uptake 180 min after termination of ingestion. Serum enhanced the cellular uptake of bacteria, but did not affect the rate of elimination. Disintegration of the phagocytes was not found to be a problem.

UI MeSH Term Description Entries
D009504 Neutrophils Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes. LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D010587 Phagocytosis The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES). Phagocytoses
D010761 Phosphorus Radioisotopes Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes. Radioisotopes, Phosphorus
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

T Midtvedt, and A Baardsen
Influence of hydrocortisone on uptake and elimination of 32P-labelled E. coli by rat polymorphonuclear neutrophils (PMN) in vitro.
April 1976, Acta pathologica et microbiologica Scandinavica. Section C, Immunology,
T Midtvedt, and A Baardsen
Phagocytosis of 32P-labelled E. coli by human polymorphonuclear cells (PMN). Adaptation of a method.
August 1978, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology,
T Midtvedt, and A Baardsen
Phagocytosis of 32P-labelled E. coli by rat peritoneal polymorphonuclear leucocytes. Evaluation of a method.
January 1968, Acta pathologica et microbiologica Scandinavica,
T Midtvedt, and A Baardsen
Effects of antibacterial agents on the elimination of 32P-labelled Escherichia coli by human polymorphonuclear cells (PMN).
April 1981, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology,
T Midtvedt, and A Baardsen
The effect of eight antibacterial agents on the elimination of 32P-labelled Escherichia coli by rat polymorphonuclear cells.
January 1977, Scandinavian journal of infectious diseases,
T Midtvedt, and A Baardsen
A study of the elimination phase of phagocytosis of 32P-labelled Escherichia coli by human polymorphonuclear cells.
February 1981, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology,
T Midtvedt, and A Baardsen
Opsonizing and 32P-releasing activities of rat intestinal strangulation fluid on 32P-labelled E. coli.
January 1969, Acta pathologica et microbiologica Scandinavica,
T Midtvedt, and A Baardsen
Degradation of non-opsonized 32P-labelled E. coli by unstimulated mouse peritoneal macrophages. Evaluation of a method.
June 1980, Acta pathologica et microbiologica Scandinavica. Section C, Immunology,
T Midtvedt, and A Baardsen
Phagocytosis of 32P-labelled Escherichia coli by human polymorphonuclear cells (PMN). Effect of different sera and PMN with reference to the ABO blood group system.
December 1979, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology,
T Midtvedt, and A Baardsen
Rat intestinal glycoprotein lowering bactericidal activity of serum on 32P-labelled E. coli.
April 1976, Acta pathologica et microbiologica Scandinavica. Section C, Immunology,
Copied contents to your clipboard!