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In order to achieve a correct diagnosis of drug anaphylaxis using a radioimmunoassay devoid of interferences, we have studied factors leading to false positive or false negative values of plasma histamine. Different steps in sample collection were studied systematically in 30 normal volunteers. False positive values were found in haemolysed samples, with histamine concentrations being correlated with haemoglobin concentrations, and where plasma was aspirated from the white-cell layer. There was no significant increase when a tourniquet or vacuum tubes were used, or when blood tubes were left at 4 degrees C overnight. In 12 patients who experienced an anaphylactic reaction, histamine disappeared from blood 10 times more slowly than expected. False negative values were found in two pregnant women and one heparinized patient. Histamine was remarkably stable in vitro in blood or plasma samples, whereas it disappeared rapidly when plasma from a pregnant woman or a heparinized patient was added to the sample. We conclude that false positive and false negative values are rare when using this radioimmunoassay.
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
November 1988,
American journal of clinical pathology,
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
December 1973,
Lancet (London, England),
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
July 1977,
Cancer research,
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
January 1985,
Head & neck surgery,
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
December 1990,
Clinical nuclear medicine,
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
October 1995,
Ugeskrift for laeger,
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
July 1982,
Arquivos brasileiros de cardiologia,
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
April 1983,
Deutsche medizinische Wochenschrift (1946),
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
January 1990,
Journal of analytical toxicology,
D Laroche, and
F Dubois, and
J L Gérard, and
C Lefrançois, and
B André, and
M C Vergnaud, and
L Dubus, and
H Bricard
June 1976,
Southern medical journal,
