Biomaterial Database

Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. 1994

W S Wun, and C C Wun, and G M Grunert

Obstetrical & Gynecological Associates, Houston, Texas 77054, USA.

OBJECTIVE Our purpose was to find a medium to enhance mouse zygote development and, hopefully, to apply the results to a coculture system and to enhance the ART pregnancy rate. METHODS The study was designed to compare different media's support of mouse zygote development with/without serum supplement. The outcome measure was the percentage of mouse zygotes/embryos that developed to the expanded blastocyst and hatchout stage. RESULTS (1) Using human tubal fluid (HTF), one-cell zygotes had a 34.6 +/- 5.2% (mean +/- standard deviation) development rate, and two-cell embryos a 86.5 +/- 3.2% development rate. (2) Minimum essential medium alpha (MEM) showed the best results (52.2 +/- 14.5%) among Ham's F-10 (19.1 +/- 6.3%), HTF (26.8 +/- 8.2%), NCTC-135 (38.8 +/- 12.6%), MEM with nuclei acid (24.6 +/- 10.0%), and Dulbecco's modified Eagle medium (28.0 +/- 20.2%). (3) With the serum supplement, there was no significant difference among Ham's F-10 (21.5 +/- 23.7), HTF (29.3 +/- 10.4%), NCTC-135 (36.5 +/- 6.2%), and MEM (38.8 +/- 17.9%). CONCLUSIONS MEM is the best medium among the six media examined. Preliminary study showed that MEM gave a good clinical pregnancy rate (29%).

UI	MeSH Term	Description	Entries
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008815	Mice, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.	Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D001755	Blastocyst	A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.	Embryo, Preimplantation,Blastocysts,Embryos, Preimplantation,Preimplantation Embryo,Preimplantation Embryos
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005260	Female		Females
D005307	Fertilization in Vitro	An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.	Test-Tube Fertilization,Fertilizations in Vitro,In Vitro Fertilization,Test-Tube Babies,Babies, Test-Tube,Baby, Test-Tube,Fertilization, Test-Tube,Fertilizations, Test-Tube,In Vitro Fertilizations,Test Tube Babies,Test Tube Fertilization,Test-Tube Baby,Test-Tube Fertilizations
D005314	Embryonic and Fetal Development	Morphological and physiological development of EMBRYOS or FETUSES.	Embryo and Fetal Development,Prenatal Programming,Programming, Prenatal
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015053	Zygote	The fertilized OVUM resulting from the fusion of a male and a female gamete.	Fertilized Ovum,Ovum, Fertilized,Fertilized Egg,Egg, Fertilized,Eggs, Fertilized,Fertilized Eggs,Zygotes