-transparent.png){kind=logo}
Whole and partially modified phosphorothioate oligodeoxynucleotides (ODN) were found to directly inhibit T4 polynucleotide kinase (PNK) activity, while phosphodiester ODN showed no detectable inhibition. This inhibition was found to be length dependent, as demonstrated by a 28-mer phosphorothioate ODN with an IC50 of 12 nM, and an 8-mer phosphorothioate ODN with an IC50 of 27,000 nM. Inhibition depended on the number and type of modified internucleotide linkages: a 20-mer phosphorothioate ODN had an IC50 of 21 nM, while a chimeric ODN with seven phosphorothioate linkages and an identical sequence showed no inhibition. On the other hand, the same sequence as a chimeric phosphorodithioate ODN (with seven dithioate linkages) had an IC50 of 580 nM. Four different chimeric phosphorodithioate ODN showed markedly different potencies of inhibition, suggesting that inhibition of PNK activity can be sequence specific.
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
January 2013,
Analytical chemistry,
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
January 1998,
Intervirology,
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
January 1986,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
November 1977,
Biochemistry,
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
January 1981,
Gene amplification and analysis,
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
May 1990,
Proceedings of the National Academy of Sciences of the United States of America,
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
February 1977,
Nucleic acids research,
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
October 1999,
International journal of cancer,
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
February 1986,
Nucleic acids research,
R M Teasdale, and
S J Matson, and
E Fisher, and
A M Krieg
January 1992,
Antisense research and development,
