Determination of serum aluminium using an ion-pair reversed-phase high-performance liquid chromatographic-fluorimetric system with lumogallion. 1995

J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
Department of Chemistry, National University of Singapore, Kent Ridge.

An ion-pair reversed-phase high-performance liquid chromatographic method with fluorimetric detection, using lumogallion [4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulphonic acid] as a ligand, has been successfully applied to the determination of aluminium in human serum. The highly fluorescent aluminium-lumogallion complex (lambda ex 505 nm, lambda em 574 nm) was separated on a LiChrosorb RP-18 column with an eluent consisting of 30% acetonitrile, 70% 0.02 M potassium hydrogen phthalate and 10 microM lumogallion. The proposed system offers a simple, rapid, selective and sensitive method for the determination of aluminium in serum. The detection limit for aluminium was 0.05 microgram/l in aqueous solution and the limit of determination was 2.2 micrograms/l in serum. The recovery of the method is generally over 90%.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D007221 Industry Any enterprise centered on the processing, assembly, production, or marketing of a line of products, services, commodities, or merchandise, in a particular field often named after its principal product. Examples include the automobile, fishing, music, publishing, insurance, and textile industries. Tertiary Sector,Industries,Sector, Tertiary,Sectors, Tertiary,Tertiary Sectors
D008024 Ligands A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed) Ligand
D008669 Metallurgy The science, art, or technology dealing with processes involved in the separation of metals from their ores, the technique of making or compounding the alloys, the techniques of working or heat-treating metals, and the mining of metals. It includes industrial metallurgy as well as metallurgical techniques employed in the preparation and working of metals used in dentistry, with special reference to orthodontic and prosthodontic appliances. (From Jablonski, Dictionary of Dentistry, 1992, p494) Heavy Industries,Industries, Heavy,Heavy Industry,Industry, Heavy
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000535 Aluminum A metallic element that has the atomic number 13, atomic symbol Al, and atomic weight 26.98. Aluminium,Aluminium-27,Aluminum-27,Aluminium 27,Aluminum 27
D001557 Benzenesulfonates Organic salts and esters of benzenesulfonic acid.
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D016273 Occupational Exposure The exposure to potentially harmful chemical, physical, or biological agents that occurs as a result of one's occupation. Exposure, Occupational,Exposures, Occupational,Occupational Exposures

Related Publications

J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
October 1989, Talanta,
J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
April 1992, Journal of chromatography,
J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
November 1990, Journal of pharmaceutical sciences,
J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
November 2009, Se pu = Chinese journal of chromatography,
J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
July 1987, Journal of chromatography,
J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
November 1989, Journal of chromatography,
J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
December 1987, Journal of chromatography,
J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
May 1992, Journal of chromatography,
J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
November 1982, Clinical chemistry,
J Wu, and C Y Zhou, and H Chi, and M K Wong, and H K Lee, and H Y Ong, and C N Ong
October 1986, Journal of chromatography,
Copied contents to your clipboard!