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A human chromosome 13-specific cosmid library has been screened with oligonucleotides containing tri- and tetranucleotide repeats with motifs: GACA, GACT, CAC, TCC. 20 cosmid clones were identified and their physical location on chromosome 13 was determined using fluorescence in situ hybridisation. Over 80% of the cosmids were detected using tetranucleotide repeats of which 50% were GACT, but these were distributed along the length of the chromosome. Clones from the 13q12-->q14 and 13q32-->q34 regions were apparently overrepresented and 10% of cosmids were localised to the short arm of the chromosome. These simple tandem repeats will be an important addition to the genetic mapping strategy for human chromosome 13.
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
January 1994,
Molekuliarnaia biologiia,
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
December 1994,
Human molecular genetics,
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
April 1995,
Human molecular genetics,
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
February 2000,
Molecular ecology,
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
January 1999,
Genetics,
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
December 2000,
Yeast (Chichester, England),
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
November 2002,
Cancer research,
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
June 1994,
Genomics,
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
February 1996,
Genomics,
L A Hawthorn, and
B Kapanadse, and
N Yankovsky, and
J K Cowell
November 1995,
BioTechniques,
