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The type and relative amounts of plasminogen activator (PA) in different fractions of bovine milk obtained from 15 Holstein cows were examined. Raw milk was centrifuged to separate skim milk and a somatic cell pellet. PA was mainly localized within the casein fraction, being 42 times that in the serum, and in association with somatic cells. The predominant form of PA in milk casein was isolated from SDS-PAGE gel extracts and had a molecular mass of approximately 75 kDa. Its activity was increased 4.1-fold (P < 0.01) in the presence of fibrin but was unaffected by the presence of amiloride, indicating that it was due to tissue-PA. The predominant forms of PA associated with milk somatic cells were isolated from SDS-PAGE gel extracts and had molecular masses of approximately 30 and approximately 50 kDa. The activity of both proteins was unaffected by the presence of fibrin but was dramatically reduced by the presence of amiloride, indicating that they represented urokinase-PA.
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
August 1998,
The Journal of dairy research,
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
June 1992,
Journal of dairy science,
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
May 1985,
Journal of dairy science,
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
August 1991,
American journal of veterinary research,
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
May 1994,
Biochimica et biophysica acta,
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
August 1980,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
April 2008,
Journal of food science,
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
July 1965,
The Biochemical journal,
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
December 1956,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
J H White, and
B Zavizion, and
K O'Hare, and
J Gilmore, and
M R Guo, and
P Kindstedt, and
I Politis
March 1960,
Thrombosis et diathesis haemorrhagica,
