Biomaterial Database

Influence of circulating D-dimer levels on assays of fibrinogen. 1994

S C Koh, and C Y Chew, and O A Viegas, and M Choo, and S S Ratnam

Department of Obstetrics and Gynaecology, National University Hospital, Singapore.

The influence of circulating D-dimer levels on two different assays for fibrinogen was studied in 31 normal female subjects, 18 late pregnant subjects, 8 male subjects with acute myocardial infarction (AMI) and 9 subjects with unstable angina (UA). Both functional and chemical methods for fibrinogen showed significant correlation (r = 0.8171; P < 0.001). However, values for fibrinogen were significantly lower using the functional method in normal females, AMI and UA patients with mean (SD) ratios of 0.88 (0.11), 0.78 (0.17) and 0.84 (0.09) respectively as compared to the chemical assay method. In late pregnancy this was not observed with a mean ratio of 1.09 (0.22) obtained between the two methods. The D-dimer level in normal subjects was mean 194 (95.3) ng/ml whilst in late pregnancy, in AMI and UA, the levels were significantly elevated at 1093 (1034), 382 (216) and 1056 (708) ng/ml respectively. Significant correlation between fibrinogen and D-dimer levels was observed only in AMI and UA patients but not in late pregnancy. In 3 patients with AMI undergoing thrombolysis, no functional fibrinogen was detectable for the first 12 h following intravenous streptokinase (SK) despite the presence of chemically detectable fibrinogen with values of 13% to 30% of pre-treatment levels and evidenced by lytic states evaluated by thromboelastography (TEG). Grossly elevated D-dimer levels of between 25 x 10(3) and over 100 x 10(3) ng/ml were present during this time. Functional fibrinogen was detectable by 24 h following SK therapy but its level in relation to fibrinogen detected chemically remained depressed.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D008180	Lupus Erythematosus, Systemic	A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.	Libman-Sacks Disease,Lupus Erythematosus Disseminatus,Systemic Lupus Erythematosus,Disease, Libman-Sacks,Libman Sacks Disease
D008297	Male		Males
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D009203	Myocardial Infarction	NECROSIS of the MYOCARDIUM caused by an obstruction of the blood supply to the heart (CORONARY CIRCULATION).	Cardiovascular Stroke,Heart Attack,Myocardial Infarct,Cardiovascular Strokes,Heart Attacks,Infarct, Myocardial,Infarction, Myocardial,Infarctions, Myocardial,Infarcts, Myocardial,Myocardial Infarctions,Myocardial Infarcts,Stroke, Cardiovascular,Strokes, Cardiovascular
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011248	Pregnancy Complications	Conditions or pathological processes associated with pregnancy. They can occur during or after pregnancy, and range from minor discomforts to serious diseases that require medical interventions. They include diseases in pregnant females, and pregnancies in females with diseases.	Adverse Birth Outcomes,Complications, Pregnancy,Adverse Birth Outcome,Birth Outcome, Adverse,Complication, Pregnancy,Outcome, Adverse Birth,Pregnancy Complication
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260	Female		Females
D005338	Fibrin Fibrinogen Degradation Products	Soluble protein fragments formed by the proteolytic action of plasmin on fibrin or fibrinogen. FDP and their complexes profoundly impair the hemostatic process and are a major cause of hemorrhage in intravascular coagulation and fibrinolysis.	Antithrombin VI,Fibrin Degradation Product,Fibrin Degradation Products,Fibrin Fibrinogen Split Products,Degradation Product, Fibrin,Degradation Products, Fibrin,Product, Fibrin Degradation
D005340	Fibrinogen	Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.	Coagulation Factor I,Factor I,Blood Coagulation Factor I,gamma-Fibrinogen,Factor I, Coagulation,gamma Fibrinogen