Detailed deletion mapping of chromosome 6q in borderline epithelial ovarian tumors. 1995

K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.

We have used PCR amplification of tandem repeats and Southern blot analysis to study the pattern of allelic loss at chromosome 6q in borderline ovarian tumors and compared that with invasive ovarian carcinomas. DNA from 46 borderline ovarian tissues, 20 invasive ovarian tumor tissues, together with corresponding uninvolved (control) tissues was used. The invasive tumors demonstrated the highest percentage of loss of heterozygosity (13 of 45 informative cases, 29%) at the 6q25-27 locus site. In contrast, the borderline ovarian tumors showed only an 11% frequency of loss of heterozygosity (3 of 26). Our results display a sharp contrast in the pattern of loss of heterozygosity between invasive and borderline ovarian tumors and suggest that allelic loss at chromosome 6q may not be involved in the development of borderline ovarian tumors.

UI MeSH Term Description Entries
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D002896 Chromosomes, Human, Pair 6 A specific pair GROUP C CHROMSOMES of the human chromosome classification. Chromosome 6
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000483 Alleles Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product. Allelomorphs,Allele,Allelomorph
D015139 Blotting, Southern A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Southern Blotting,Blot, Southern,Southern Blot
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D017353 Gene Deletion A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus. Deletion, Gene,Deletions, Gene,Gene Deletions

Related Publications

K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
October 1995, Oncogene,
K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
October 1996, Cancer research,
K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
June 2008, Pediatrics and neonatology,
K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
November 1998, Oncogene,
K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
January 1998, Oncogene,
K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
September 2000, British journal of cancer,
K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
November 1990, Gynecologic oncology,
K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
February 1992, Rinsho byori. The Japanese journal of clinical pathology,
K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
August 1996, Zhonghua yi xue za zhi = Chinese medical journal; Free China ed,
K J Rodabaugh, and G Blanchard, and W R Welch, and D A Bell, and R S Berkowitz, and S C Mok
November 1999, Zhonghua zhong liu za zhi [Chinese journal of oncology],
Copied contents to your clipboard!