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Factors and cells of placental origin have been considered to be important in mediating local active immunosuppression that regulates maternal immune reactivity to aid fetal survival. In this context, we investigated the immunosuppressive capabilities of supernatants from human trophoblast-derived choriocarcinoma cell lines (HCS). We have previously reported the inhibitory effects of HCS on proliferative responses of T-lymphocytes both in vitro and in vivo (L. Krishnan, E. Menu, G. Chaouat, G. P. Talwar, and R. Raghupathy, Cell. Immunol. 138, 113, 1991; L. Krishnan, R. Kinsky, G. Chaouat, G.P. Talwar, and R. Raghupathy, Cell. Immunol. 150, 376, 1993). We now show that HCS also suppresses LPS-induced proliferation of murine lymphocytes but does not inhibit the constitutive proliferation of lymphoma cell lines and B cell hybridomas indicating that HCS has no inhibitory effects on terminally differentiated or transformed cells. Furthermore, we have succeeded in isolating and partially characterizing the HCS-derived suppressor factor (HCSf) from culture supernatants of a human choriocarcinoma cell line JEG-3. Purification of the factor has been accomplished by sequential fractionation on anion-exchange, gel filtration, and reverse-phase HPLC columns. The suppressor factor is a low-molecular-weight compound in the range of 5-6 kDa, composed predominantly of hydrophilic amino acids. Protease digestion of the factor revealed that the peptide moiety in HCSf is important for its inhibitory activity. HCSf mediates a dose-dependent suppression of proliferative responses of both human and murine lymphocytes.
L Krishnan, and
S Sad, and
R Raghupathy
November 1997,
American journal of reproductive immunology (New York, N.Y. : 1989),
L Krishnan, and
S Sad, and
R Raghupathy
May 2001,
Placenta,
L Krishnan, and
S Sad, and
R Raghupathy
March 1986,
Biochemical and biophysical research communications,
L Krishnan, and
S Sad, and
R Raghupathy
January 1989,
Journal of Tongji Medical University = Tong ji yi ke da xue xue bao,
L Krishnan, and
S Sad, and
R Raghupathy
June 1986,
Nihon Sanka Fujinka Gakkai zasshi,
L Krishnan, and
S Sad, and
R Raghupathy
January 1987,
The Journal of biological chemistry,
L Krishnan, and
S Sad, and
R Raghupathy
January 1991,
Preparative biochemistry,
L Krishnan, and
S Sad, and
R Raghupathy
August 1992,
International journal of hematology,
L Krishnan, and
S Sad, and
R Raghupathy
March 1991,
Japanese journal of cancer research : Gann,
L Krishnan, and
S Sad, and
R Raghupathy
November 2001,
American journal of reproductive immunology (New York, N.Y. : 1989),
