Biomaterial Database

Development of single blastomeres from 4-cell stage embryos after aggregation with parthenogenones in mice. 1994

A Pinyopummin, and Y Takahashi, and M Hishinuma, and H Kanagawa

Department of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

This study was undertaken to determine whether diploid parthenogenones can assist the development of single blastomeres of 4-cell embryos to term. Isolated blastomeres of 4-cell embryos were aggregated with parthenogenones at the 2-, 4- and 8-cell stages. After the removal of the zonae, aggregation was done by pushing one blastomere of 4-cell embryos and one parthenogenone into contact using a micropipette. There were no significant differences in the percentages of blastocysts and offspring among all parthenogenones' developmental stages used. It also seemed that the chimerism of the offspring (judged by coat color) was not affected by the developmental stage of the parthenogenone. A total of 19 offspring survived and 12 offspring were coat-colored chimera. From 12 coat-colored chimeric offspring, 9 offspring had germlines derived from the isolated blastomeres of 4-cell embryos, one offspring had a germline derived from both the parthenogenone and the isolated blastomere of a 4-cell embryo and one offspring had a germline derived from the parthenogenone. One offspring however, was infertile. The present study demonstrates that parthenogenones can be used to assist the development of single blastomeres from 4-cell embryos to term.

UI	MeSH Term	Description	Entries
D008808	Mice, Inbred CBA	An inbred strain of mouse that is widely used in BIOMEDICAL RESEARCH.	Mice, CBA,Mouse, CBA,Mouse, Inbred CBA,CBA Mice,CBA Mice, Inbred,CBA Mouse,CBA Mouse, Inbred,Inbred CBA Mice,Inbred CBA Mouse
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008813	Mice, Inbred ICR	An inbred strain of mouse that is used as a general purpose research strain, for therapeutic drug testing, and for the genetic analysis of CARCINOGEN-induced COLON CANCER.	Mice, Inbred ICRC,Mice, ICR,Mouse, ICR,Mouse, Inbred ICR,Mouse, Inbred ICRC,ICR Mice,ICR Mice, Inbred,ICR Mouse,ICR Mouse, Inbred,ICRC Mice, Inbred,ICRC Mouse, Inbred,Inbred ICR Mice,Inbred ICR Mouse,Inbred ICRC Mice,Inbred ICRC Mouse
D010312	Parthenogenesis	A unisexual reproduction without the fusion of a male and a female gamete (FERTILIZATION). In parthenogenesis, an individual is formed from an unfertilized OVUM that did not complete MEIOSIS. Parthenogenesis occurs in nature and can be artificially induced.	Arrhenotoky,Automixis,Thelytoky,Parthenogeneses
D001757	Blastomeres	Undifferentiated cells resulting from cleavage of a fertilized egg (ZYGOTE). Inside the intact ZONA PELLUCIDA, each cleavage yields two blastomeres of about half size of the parent cell. Up to the 8-cell stage, all of the blastomeres are totipotent. The 16-cell MORULA contains outer cells and inner cells.	Blastocytes,Blastocyte,Blastomere
D002678	Chimera	An individual that contains cell populations derived from different zygotes.	Hybrids,Chimeras,Hybrid
D002970	Cleavage Stage, Ovum	The earliest developmental stage of a fertilized ovum (ZYGOTE) during which there are several mitotic divisions within the ZONA PELLUCIDA. Each cleavage or segmentation yields two BLASTOMERES of about half size of the parent cell. This cleavage stage generally covers the period up to 16-cell MORULA.	Segmentation Stage, Ovum,Cleavage Stages, Ovum,Ovum Cleavage Stage,Ovum Cleavage Stages,Ovum Segmentation Stage,Ovum Segmentation Stages,Segmentation Stages, Ovum
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus