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In a series of in vitro experiments we characterised the relationship between DNA distribution in the G1, S and G2/M phases of cell cycle and PDE and GST activity in CaCo-2 cells. The DNA distribution in CaCo-2 cells, was assessed by flow cytometry, with fluorescent dyes at different time points of culture. The exponential increase in cell number continued until day 10 when there was cell saturation. The effect of medium replacement on PDE activity was assayed in the first 10 h after medium replacement. The 6th hour is the time at which PDE activity was found to be highest. We have assayed the PDE enzyme with cGMP and cAMP as substrates. Only cAMP was consumed from this enzyme. We found a very close correlation between the DNA distribution in the various phases of the cell cycle and the PDE activity. PDE activity was very high during the active replication phase, whereas GST activity was high after confluency.
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
July 2000,
Biological & pharmaceutical bulletin,
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
October 1989,
Gastroenterology,
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
January 1990,
Digestion,
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
April 1990,
Infection and immunity,
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
May 1990,
Journal of cellular physiology,
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
February 1999,
Toxicology in vitro : an international journal published in association with BIBRA,
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
March 2011,
Toxicology in vitro : an international journal published in association with BIBRA,
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
January 1985,
Biology of the cell,
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
January 2000,
Annals of the New York Academy of Sciences,
F Savini, and
S Berardi, and
D Tatone, and
G Spoto
January 2000,
Digestion,
