Biomaterial Database

Alkaline phosphatase activity in cultured cells of human healthy and hyperplastic gingivae: a preliminary study. 1994

L T Hou, and C M Liu, and W K Chang

School of Dentistry, Graduate Institute of Oral Sciences, National Taiwan University, Taipei, R.O.C.

The present study compared the alkaline phosphatase (ALP) expression in cultured cells derived from different types of gingival hyperplasia. Tissue biopsies were obtained from patients with clinically non-inflamed, enlarged gingivae of idiopathic gingivofibromatosis (IGF) and phenytoin-induced hyperplasia (PHG). Fibroblasts obtained from the gingivae or periodontal ligaments (PDL) of the same or different healthy subjects were used as controls. Fibroblasts were plated and the ALP activity and total amount of DNA per well were studied in each isolated cell type at 4, 7 and 9 days of culture. The results revealed that fibroblasts derived from IGF tissue synthesized a large amount of ALP by day 7 and day 9 of culture. The amount of ALP activity was cell density-dependent and the strongest expression of enzyme activity was noted after cell confluence. In contrast, only very low ALP activity was detected in cells derived from normal gingivae and PHG. Cytochemical examination of these cells revealed similar observations. The fact that fibroblasts derived from PHG tissue did not exhibit ALP in vitro suggests that there was a shift of cell phenotype in this tissue. The increased expression of ALP in IGF cells may imply a developmental trend toward osteoblastic phenotypes.

UI	MeSH Term	Description	Entries
D010672	Phenytoin	An anticonvulsant that is used to treat a wide variety of seizures. It is also an anti-arrhythmic and a muscle relaxant. The mechanism of therapeutic action is not clear, although several cellular actions have been described including effects on ion channels, active transport, and general membrane stabilization. The mechanism of its muscle relaxant effect appears to involve a reduction in the sensitivity of muscle spindles to stretch. Phenytoin has been proposed for several other therapeutic uses, but its use has been limited by its many adverse effects and interactions with other drugs.	Diphenylhydantoin,Fenitoin,Phenhydan,5,5-Diphenylhydantoin,5,5-diphenylimidazolidine-2,4-dione,Antisacer,Difenin,Dihydan,Dilantin,Epamin,Epanutin,Hydantol,Phenytoin Sodium,Sodium Diphenylhydantoinate,Diphenylhydantoinate, Sodium
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D005351	Fibromatosis, Gingival	Generalized or localized diffuse fibrous overgrowth of the gingival tissue, usually transmitted as an autosomal dominant trait, but some cases are idiopathic and others produced by drugs. The enlarged gingiva is pink, firm, and has a leather-like consistency with a minutely pebbled surface and in severe cases the teeth are almost completely covered and the enlargement projects into the oral vestibule. (Dorland, 28th ed)	Gingival Fibromatosis,Fibromatosis Gingivae,Fibromatoses, Gingival,Gingival Fibromatoses
D005881	Gingiva	Oral tissue surrounding and attached to TEETH.	Gums,Interdental Papilla,Papilla, Interdental,Gum
D005885	Gingival Hyperplasia	Non-inflammatory enlargement of the gingivae produced by factors other than local irritation. It is characteristically due to an increase in the number of cells. (From Jablonski's Dictionary of Dentistry, 1992, p400)	Gingival Hyperplasias,Hyperplasia, Gingival,Hyperplasias, Gingival
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000469	Alkaline Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.