Antigen-antibody diffusion-limited binding kinetics of biosensors: a fractal analysis. 1995

A Sadana, and A M Beelaram
Chemical Engineering Department, University of Mississippi 38677-9740, USA.

A fractal analysis is made of antigen-antibody binding kinetics in various biosensor applications reported in the literature. Both sensor configurations are considered, wherein (a) the antigen is in solution and the antibody is immobilized on the fibre-optic surface, and (b) the antibody is in solution and the antigen is immobilized on the fibre-optic surface. For example, when the antigen is immobilized, an increase in the antibody concentration (anti-Ars) in solution leads to (a) an increase in the fractal dimension value and hence the state of disorder or roughness, and (b) a higher rate coefficient for binding on the fibre-optic surface. An analysis of the influence of different parameters on the fractal dimension values for a particular effect, such as a comparison of fresh and regenerated immunosensors, helps to clarify the conformational states and reactions occurring on the fibre-optic surface. Examination of the different cases presented together provides novel physical insights into the state of disorder or roughness on the surface. This research should contribute towards techniques for manipulating the reactions occurring on fibre-optic surfaces in desired directions.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008962 Models, Theoretical Theoretical representations that simulate the behavior or activity of systems, processes, or phenomena. They include the use of mathematical equations, computers, and other electronic equipment. Experimental Model,Experimental Models,Mathematical Model,Model, Experimental,Models (Theoretical),Models, Experimental,Models, Theoretic,Theoretical Study,Mathematical Models,Model (Theoretical),Model, Mathematical,Model, Theoretical,Models, Mathematical,Studies, Theoretical,Study, Theoretical,Theoretical Model,Theoretical Models,Theoretical Studies
D000937 Antigen-Antibody Reactions The processes triggered by interactions of ANTIBODIES with their ANTIGENS. Antigen Antibody Reactions,Antigen-Antibody Reaction,Reaction, Antigen-Antibody,Reactions, Antigen-Antibody
D015374 Biosensing Techniques Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal. Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing

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