Biomaterial Database

The effect of discontinuous Percoll gradients preparation on sperm movement characteristics. 1994

N Sukcharoen

Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

To determine the improvement of sperm movement characteristics after discontinuous Percoll gradients preparation, sperm movement characteristics from 60 normal semen samples were assessed before and after discontinuous Percoll gradients preparation using Hamilton-Thorn Motility Analyzer (HTMA). There was a significant correlation between manual and HTMA results for the percentage of motile sperm (r = 0.8670, p < 0.001). The percentage rapid, percentage motile, average path velocity (VAP), progressive velocity (VSL), curvilinear velocity (VCL), beat cross frequency (BCF), amplitude of head displacement (ALH), percentage of progressive motility, and hyperactivation were significantly increased after preparation. Therefore, this sperm preparation technique is a convenient and efficient method to prepare progressive motile sperm for assisted conceptive technology.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D002470	Cell Survival	The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.	Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002498	Centrifugation	Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013048	Specimen Handling	Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.	Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D013081	Sperm Motility	Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.	Motilities, Sperm,Motility, Sperm,Sperm Motilities